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  1. Link

    idu-antibody-oti2f8-ab187742.pdf

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Anti-IdU antibody [OTI2F8] (ab187742)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (1)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IdU antibody [OTI2F8] (ab187742)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IdU antibody [OTI2F8] (ab187742)

Key features and details

  • Rat monoclonal [OTI2F8] to IdU
  • Suitable for: IHC-P
  • Isotype: IgG2a

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Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
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Alexa Fluor® 488 Anti-BrdU antibody [BU1/75 (ICR1)] (ab220074)

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Overview

  • Product name

    Anti-IdU antibody [OTI2F8]
  • Description

    Rat monoclonal [OTI2F8] to IdU
  • Host species

    Rat
  • Specificity

    ab187742 has been shown to cross react with BrdU. Cross-reactivity with other similar molecules has not been tested.

  • Tested applications

    Suitable for: IHC-Pmore details
  • Immunogen

    Chemical/ Small Molecule corresponding to IdU conjugated to Keyhole Limpet Haemocyanin (KLH).

  • Positive control

    • Colon tissue from IdU injected mouse.
  • General notes

    The clone number has been updated from 2F8 to OTI2F8, both clone numbers name the same clone.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 48% PBS, 50% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    ab187742 is purified from cell culture supernatant by affinity chromatography.
  • Clonality

    Monoclonal
  • Clone number

    OTI2F8
  • Isotype

    IgG2a
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Markers
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA / Nucleotides

Associated products

  • Compatible Secondaries

    • Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
    • Goat Anti-Rat IgG H&L (HRP) (ab205720)
  • Isotype control

    • Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control (ab18450)
  • Related Products

    • Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (ab6326)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab187742 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/150.
Notes
IHC-P
1/150.

Target

  • Relevance

    Iododeoxyuridine (5-Iodo-2'-deoxyuridine, IdU) is a synthetic thymidine nucleoside analog. IdU, like BrdU thymidine analog, is used to allow the detection of growing or proliferating cells in living tissues. During the S-phase of cell division, DNA replication occurs, and IdU can be incorporated into the newly synthesized DNA by substituting for naturally occurring thymidine. Antibodies specific for IdU are subsequently used to detect the incorporated IdU thymidine analog. This highlights cells that were actively replicating their DNA and is suggestive of actively growing cells. Antibody binding usually requires the DNA to be denatured, typically by exposing the cells to acid or heat.
  • Alternative names

    • 5-Iodo-2'-deoxyuridine antibody
    • Iododeoxyuridine antibody

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IdU antibody [OTI2F8] (ab187742)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IdU antibody [OTI2F8] (ab187742)

    Immunohistochemical analysis of paraffin-embedded colon tissue from IdU injected mouse, labeling IdU with ab187742 at 1/150 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IdU antibody [OTI2F8] (ab187742)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IdU antibody [OTI2F8] (ab187742)

    Immunohistochemical analysis of paraffin-embedded colon tissue from BrdU injected mouse, labeling IdU with ab187742 at 1/150 dilution.

Protocols

  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1)

Publishing research using ab187742? Please let us know so that we can cite the reference in this datasheet.

ab187742 has been referenced in 1 publication.

  • Smith R  et al. Poly(ADP-ribose)-dependent chromatin unfolding facilitates the association of DNA-binding proteins with DNA at sites of damage. Nucleic Acids Res 47:11250-11267 (2019). PubMed: 31566235

Customer reviews and Q&As

Show All Reviews Q&A
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IHC - Wholemount abreview for Anti-IdU antibody [OTI2F8]

Excellent
Abreviews
Abreviews
abreview image
Application
IHC - Wholemount
Sample
Mouse Tissue (Cornea)
Specification
Cornea
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Dec 17 2019

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