Key features and details
- Rat monoclonal [OTI2F8] to IdU
- Suitable for: IHC-P
- Isotype: IgG2a
Product nameAnti-IdU antibody [OTI2F8]
DescriptionRat monoclonal [OTI2F8] to IdU
ab187742 has been shown to cross react with BrdU. Cross-reactivity with other similar molecules has not been tested.
Tested applicationsSuitable for: IHC-Pmore details
Chemical/ Small Molecule corresponding to IdU conjugated to Keyhole Limpet Haemocyanin (KLH).
- Colon tissue from IdU injected mouse.
The clone number has been updated from 2F8 to OTI2F8, both clone numbers name the same clone.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 48% PBS, 50% Glycerol, 1% BSA
Concentration information loading...
PurityProtein A purified
Purification notesab187742 is purified from cell culture supernatant by affinity chromatography.
Our Abpromise guarantee covers the use of ab187742 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
RelevanceIododeoxyuridine (5-Iodo-2'-deoxyuridine, IdU) is a synthetic thymidine nucleoside analog. IdU, like BrdU thymidine analog, is used to allow the detection of growing or proliferating cells in living tissues. During the S-phase of cell division, DNA replication occurs, and IdU can be incorporated into the newly synthesized DNA by substituting for naturally occurring thymidine. Antibodies specific for IdU are subsequently used to detect the incorporated IdU thymidine analog. This highlights cells that were actively replicating their DNA and is suggestive of actively growing cells. Antibody binding usually requires the DNA to be denatured, typically by exposing the cells to acid or heat.
- 5-Iodo-2'-deoxyuridine antibody
- Iododeoxyuridine antibody
ab187742 has been referenced in 1 publication.
- Smith R et al. Poly(ADP-ribose)-dependent chromatin unfolding facilitates the association of DNA-binding proteins with DNA at sites of damage. Nucleic Acids Res 47:11250-11267 (2019). PubMed: 31566235