Key features and details
- Mouse polyclonal to Iduronate 2 sulfatase/SIDS
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Iduronate 2 sulfatase/SIDS antibody
See all Iduronate 2 sulfatase/SIDS primary antibodies
DescriptionMouse polyclonal to Iduronate 2 sulfatase/SIDS
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Full length protein corresponding to Human Iduronate 2 sulfatase/SIDS aa 1-550.
Database link: NP_000193.1
- Iduronate 2 sulfatase/SIDS transfected 293T lysate.
This product was previously labelled as Iduronate 2 sulfatase
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.4
Constituent: 2.68% PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab70025 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 62 kDa).|
FunctionRequired for the lysosomal degradation of heparan sulfate and dermatan sulfate.
Tissue specificityLiver, kidney, lung, and placenta.
Involvement in diseaseDefects in IDS are the cause of mucopolysaccharidosis type 2 (MPS2) [MIM:309900]; also known as Hunter syndrome. MPS2 is an X-linked lysosomal storage disease characterized by intracellular accumulation of heparan sulfate and dermatan sulfate and their excretion in urine. Most children with MPS2 have a severe form with early somatic abnormalities including skeletal deformities, hepatosplenomegaly, and progressive cardiopulmonary deterioration. A prominent feature is neurological damage that presents as developmental delay and hyperactivity but progresses to mental retardation and dementia. They die before 15 years of age, usually as a result of obstructive airway disease or cardiac failure. In contrast, those with a mild form of MPS2 may survive into adulthood, with attenuated somatic complications and often without mental retardation.
Sequence similaritiesBelongs to the sulfatase family.
modificationsThe conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity.
- Information by UniProt
- Alpha L iduronate sulfate sulfatase antibody
- Alpha-L-iduronate sulfate sulfatase antibody
- AW214631 antibody
All lanes : Anti-Iduronate 2 sulfatase/SIDS antibody (ab70025) at 1/500 dilution
Lane 1 : Iduronate 2 sulfatase/SIDS transfected 293T lysate
Lane 2 : Non transfected 293T lysate
Lysates/proteins at 25 µg per lane.
Lane 1 : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Lane 2 : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size: 62 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
ab70025 has not yet been referenced specifically in any publications.