Overview

  • Product name

    Anti-IER3IP1 antibody [EPR13440]
    See all IER3IP1 primary antibodies
  • Description

    Rabbit monoclonal [EPR13440] to IER3IP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, IHC-P, WB, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human IER3IP1 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9Y5U9

  • Positive control

    • HepG2, HeLa and Human fetal heart lysates; Human kidney and Human cardiac muscle tissues; HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR13440
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181247 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/210.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/100.
WB 1/1000. Detects a band of approximately 9 kDa (predicted molecular weight: 9 kDa).
ICC/IF 1/100 - 1/250.
IP 1/50 - 1/70.

Target

Images

  • All lanes : Anti-IER3IP1 antibody [EPR13440] (ab181247) at 1/1000 dilution

    Lane 1 : HepG2 lysate
    Lane 2 : HeLa lysate
    Lane 3 : Human fetal heart lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 9 kDa

  • Immunofluorescent analysis of HeLa cells (-20°C Acetone-fixed) labeling IER3IP1 with ab181247 at 1/250 dilution, dollowed by Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 dilution and DAPI staining (blue).

  • Western blot analysis of immunoprecipitation pellet from HeLa cell lysate  immunoprecipitated using ab181247 at 1/50 dilution.
    Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000.

  • Flow Cytometricl analysis of HeLa cells (paraformaldehyde-fixed, 2%) labeling IER3IP1 with ab181247 at 1/210 (red) compared to a negative control antibody (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling IER3IP1 with ab181247 at 1/100 dilution, followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling IER3IP1 with ab181247 at 1/100 dilution, followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

References

This product has been referenced in:

  • Mao MH  et al. Additive antitumor effect of arsenic trioxide combined with intravesical bacillus Calmette-Guerin immunotherapy against bladder cancer through blockade of the IER3/Nrf2 pathway. Biomed Pharmacother 107:1093-1103 (2018). Read more (PubMed: 30257321) »
See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab181247.
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