Anti-IEX1/IER3 antibody (ab65152)
Key features and details
- Rabbit polyclonal to IEX1/IER3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-IEX1/IER3 antibody -
Description
Rabbit polyclonal to IEX1/IER3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human adrenal tissue. ICC/IF: Human brain cells. WB: Human brain tissue lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab65152 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 17 kDa.
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IHC-P |
Use a concentration of 5 µg/ml.
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ICC/IF |
Use a concentration of 20 µg/ml.
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Notes |
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WB
Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 17 kDa. |
IHC-P
Use a concentration of 5 µg/ml. |
ICC/IF
Use a concentration of 20 µg/ml. |
Target
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Function
May play a role in the ERK signaling pathway by inhibiting the dephosphorylation of ERK by phosphatase PP2A-PPP2R5C holoenzyme. Acts also as an ERK downstream effector mediating survival. As a member of the NUPR1/RELB/IER3 survival pathway, may provide pancreatic ductal adenocarcinoma with remarkable resistance to cell stress, such as starvation or gemcitabine treatment. -
Sequence similarities
Belongs to the IER3 family. -
Post-translational
modificationsPhosphorylated at Thr-18, Thr-123 and Ser-126 by MAPK1/ERK2 and probably MAPK3/ERK1. Upon phosphorylation by MAPK1/ERK2 and MAPK3/ERK1, acquires the ability to inhibit cell death induced by various stimuli.
Glycosylated. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 8870 Human
- Entrez Gene: 15937 Mouse
- Omim: 602996 Human
- SwissProt: P46695 Human
- SwissProt: P46694 Mouse
- Unigene: 730861 Human
- Unigene: 76095 Human
- Unigene: 25613 Mouse
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Alternative names
- DIF2 antibody
- Differentiation-dependent gene 2 protein antibody
- GLY96 antibody
see all
Images
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human adrenal tissue, staining IEX1/IER3 with ab65152 at 5 µg/ml.
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Lane 1 : Anti-IEX1/IER3 antibody (ab65152) at 2 µg/ml
Lane 2 : Anti-IEX1/IER3 antibody (ab65152) at 4 µg/ml
All lanes : Human brain tissue lysate
Predicted band size: 17 kDa -
Immunocytochemistry/ Immunofluorescence analysis of human brain cells staining IEX1/IER3 with ab65152 at 20 µg/ml.
Protocols
Datasheets and documents
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Datasheet download
References (6)
ab65152 has been referenced in 6 publications.
- Zhou Q et al. Dysregulated IER3 Expression is Associated with Enhanced Apoptosis in Titin-Based Dilated Cardiomyopathy. Int J Mol Sci 18:N/A (2017). PubMed: 28353642
- Shahid M et al. IEX-1 deficiency induces browning of white adipose tissue and resists diet-induced obesity. Sci Rep 6:24135 (2016). IHC . PubMed: 27063893
- Wang YP et al. Identification of immediate early gene X-1 as a cellular target gene of hcmv-mir-UL148D. Int J Mol Med 31:959-66 (2013). PubMed: 23403649
- De Luisi A et al. Lenalidomide restrains motility and overangiogenic potential of bone marrow endothelial cells in patients with active multiple myeloma. Clin Cancer Res 17:1935-46 (2011). PubMed: 21307145
- Ria R et al. Gene expression profiling of bone marrow endothelial cells in patients with multiple myeloma. Clin Cancer Res 15:5369-78 (2009). WB ; Human . PubMed: 19690192
- Bruheim S et al. Gene expression profiles classify human osteosarcoma xenografts according to sensitivity to doxorubicin, cisplatin, and ifosfamide. Clin Cancer Res 15:7161-9 (2009). WB ; Mouse . PubMed: 19920113