Anti-IEX1/IER3 antibody - N-terminal (ab170453)
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Overview
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Product name
Anti-IEX1/IER3 antibody - N-terminal
See all IEX1/IER3 primary antibodies -
Description
Rabbit polyclonal to IEX1/IER3 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human IEX1/IER3 aa 45-73 (N terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: P46695 -
Positive control
- WB: A549 and MCF-7 cell lysates. Mouse heart and lung tissue lysate. Flow Cyt: A549 cells. ICC/IF: A549 cells. IHC-P: Human liver tissue.
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General notes
This product was previously labelled as IEX1
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab170453 was purified through a protein A column, followed by peptide affinity purification. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab170453 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/100 - 1/500. Predicted molecular weight: 17 kDa. | |
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| Flow Cyt | 1/10 - 1/50. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
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| ICC/IF | 1/10 - 1/50. |
Target
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Function
May play a role in the ERK signaling pathway by inhibiting the dephosphorylation of ERK by phosphatase PP2A-PPP2R5C holoenzyme. Acts also as an ERK downstream effector mediating survival. As a member of the NUPR1/RELB/IER3 survival pathway, may provide pancreatic ductal adenocarcinoma with remarkable resistance to cell stress, such as starvation or gemcitabine treatment. -
Sequence similarities
Belongs to the IER3 family. -
Post-translational
modificationsPhosphorylated at Thr-18, Thr-123 and Ser-126 by MAPK1/ERK2 and probably MAPK3/ERK1. Upon phosphorylation by MAPK1/ERK2 and MAPK3/ERK1, acquires the ability to inhibit cell death induced by various stimuli.
Glycosylated. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 8870 Human
- Entrez Gene: 15937 Mouse
- Omim: 602996 Human
- SwissProt: P46695 Human
- SwissProt: P46694 Mouse
- Unigene: 730861 Human
- Unigene: 76095 Human
- Unigene: 25613 Mouse
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Alternative names
- DIF2 antibody
- Differentiation-dependent gene 2 protein antibody
- GLY96 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-IEX1/IER3 antibody - N-terminal (ab170453)Confocal immunofluorescent analysis of A549 cells using ab170453 at a 1/10 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Western blot - Anti-IEX1/IER3 antibody - N-terminal (ab170453)Anti-IEX1/IER3 antibody - N-terminal (ab170453) at 1/2000 dilution + Mouse lung cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size: 17 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IEX1/IER3 antibody - N-terminal (ab170453)Immunohistochemical analysis of paraffin embedded Human liver tissue labeling IEX1/IER3 with ab170453 at a 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot - Anti-IEX1/IER3 antibody - N-terminal (ab170453)Anti-IEX1/IER3 antibody - N-terminal (ab170453) at 1/1000 dilution + Mouse heart cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size: 17 kDa -
Flow Cytometry - Anti-IEX1/IER3 antibody - N-terminal (ab170453)Flow cytometric analysis of A549 cells (green) compared to a negative control cell (blue) using ab170453 at a 1/10 dilution. FITC conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot - Anti-IEX1/IER3 antibody - N-terminal (ab170453)Anti-IEX1/IER3 antibody - N-terminal (ab170453) at 1/1000 dilution + MCF-7 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size: 17 kDa -
Western blot - Anti-IEX1/IER3 antibody - N-terminal (ab170453)Anti-IEX1/IER3 antibody - N-terminal (ab170453) at 1/100 dilution + A549 cell line lysate at 35 µg
Predicted band size: 17 kDa -
Western blot - Anti-IEX1/IER3 antibody - N-terminal (ab170453)Anti-IEX1/IER3 antibody - N-terminal (ab170453) at 1/100 dilution + Mouse heart tissue lysate at 35 µg
Predicted band size: 17 kDa
Protocols
Datasheets and documents
References
ab170453 has not yet been referenced specifically in any publications.
