Overview

  • Product name

    Anti-IEX1/IER3 antibody - N-terminal
    See all IEX1/IER3 primary antibodies
  • Description

    Rabbit polyclonal to IEX1/IER3 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human IEX1/IER3 aa 45-73 (N terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: P46695

  • Positive control

    • WB: A549 and MCF-7 cell lysates. Mouse heart and lung tissue lysate. Flow Cyt: A549 cells. ICC/IF: A549 cells. IHC-P: Human liver tissue.
  • General notes

     This product was previously labelled as IEX1

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab170453 was purified through a protein A column, followed by peptide affinity purification.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170453 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 17 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/10 - 1/50.

Target

  • Function

    May play a role in the ERK signaling pathway by inhibiting the dephosphorylation of ERK by phosphatase PP2A-PPP2R5C holoenzyme. Acts also as an ERK downstream effector mediating survival. As a member of the NUPR1/RELB/IER3 survival pathway, may provide pancreatic ductal adenocarcinoma with remarkable resistance to cell stress, such as starvation or gemcitabine treatment.
  • Sequence similarities

    Belongs to the IER3 family.
  • Post-translational
    modifications

    Phosphorylated at Thr-18, Thr-123 and Ser-126 by MAPK1/ERK2 and probably MAPK3/ERK1. Upon phosphorylation by MAPK1/ERK2 and MAPK3/ERK1, acquires the ability to inhibit cell death induced by various stimuli.
    Glycosylated.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • DIF2 antibody
    • Differentiation-dependent gene 2 protein antibody
    • GLY96 antibody
    • IER3 antibody
    • IEX1_HUMAN antibody
    • IEX1L antibody
    • Immediate early protein GLY96 antibody
    • Immediate early response 3 antibody
    • Immediate early response 3 protein antibody
    • Immediately early gene X 1 antibody
    • PACAP-responsive gene 1 protein antibody
    • PRG 1 antibody
    • PRG1 antibody
    • Protein DIF 2 antibody
    • Protein DIF-2 antibody
    • Protein PRG1 antibody
    • Radiation-inducible immediate-early gene IEX-1 antibody
    see all

Images

  • Confocal immunofluorescent analysis of A549 cells using ab170453 at a 1/10 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

  • Anti-IEX1/IER3 antibody - N-terminal (ab170453) at 1/2000 dilution + Mouse lung cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

    Predicted band size: 17 kDa

  • Immunohistochemical analysis of paraffin embedded Human liver tissue labeling IEX1/IER3 with ab170453 at a 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Anti-IEX1/IER3 antibody - N-terminal (ab170453) at 1/1000 dilution + Mouse heart cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

    Predicted band size: 17 kDa

  • Flow cytometric analysis of A549 cells (green) compared to a negative control cell (blue) using ab170453 at a 1/10 dilution. FITC conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • Anti-IEX1/IER3 antibody - N-terminal (ab170453) at 1/1000 dilution + MCF-7 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

    Predicted band size: 17 kDa

  • Anti-IEX1/IER3 antibody - N-terminal (ab170453) at 1/100 dilution + A549 cell line lysate at 35 µg

    Predicted band size: 17 kDa

  • Anti-IEX1/IER3 antibody - N-terminal (ab170453) at 1/100 dilution + Mouse heart tissue lysate at 35 µg

    Predicted band size: 17 kDa

References

ab170453 has not yet been referenced specifically in any publications.

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