Overview

  • Product name
  • Description
    Rabbit polyclonal to IF3EI
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Cow, Human, Xenopus laevis, Xenopus tropicalis
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 330-564 of Human IF3EI (UniProt Q9Y262).

  • Positive control
    • Mouse brown adipose and white adipose whole cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 79% PBS, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154527 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 66 kDa.

Target

  • Function
    Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
  • Sequence similarities
    Belongs to the eIF-3 subunit L family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • eIEF associated protein HSPC021 antibody
    • EIF3EIP antibody
    • eif3l antibody
    • EIF3L_HUMAN antibody
    • EIF3S11 antibody
    • EIF3S6IP antibody
    • Eukaryotic translation initiation factor 3 subunit 6 interacting antibody
    • Eukaryotic translation initiation factor 3 subunit 6-interacting protein antibody
    • Eukaryotic translation initiation factor 3 subunit E-interacting protein antibody
    • Eukaryotic translation initiation factor 3 subunit L antibody
    • eukaryotic translation initiation factor 3, subunit E interacting antibody
    • HSPC021 antibody
    • HSPC025 antibody
    • MSTP005 antibody
    see all

Images

  • All lanes : Anti-IF3EI antibody (ab154527) at 1/1000 dilution

    Lane 1 : Mouse brown adipose whole cell lysate
    Lane 2 : Mouse white adipose whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Predicted band size: 66 kDa



    7.5% SDS PAGE

References

ab154527 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab154527.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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