Recombinant Anti-IFI16 antibody [EPR11767(B)] (ab169788)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] (ab169788)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR11767(B)] to IFI16
Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P, WB
Reacts with: Human

Carrier Free

Product name

Anti-IFI16 antibody [EPR11767(B)]
See all IFI16 primary antibodies
Description

Rabbit monoclonal [EPR11767(B)] to IFI16
Host species

Rabbit
Tested applications

Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P, WBmore details
Unsuitable for: IP
Species reactivity

Reacts with: Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Purchase matching WB positive control:Recombinant Human IFI16 protein
- Recombinant Human IFI16 protein (ab158724) can be used as a positive control in WB. Human tonsil and bone marrow tissues. Raji cells. IFN gamma-treated U937, U937, Jurkat and Raji cell lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR11767(B)
Isotype

IgG
Research areas

Alternative Versions
- Anti-IFI16 antibody [EPR11767(B)] - BSA and Azide free (ab249529)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
Recombinant Protein
- Recombinant Human IFI16 protein (ab158724)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab169788 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
Flow Cyt (Intra)		1/500.
ICC/IF		1/100 - 1/250.
IHC-P		1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. The use of an HRP/AP polymerized secondary antibody is recommended.
WB		1/1000 - 1/10000. Predicted molecular weight: 88 kDa.

Notes
Flow Cyt (Intra) 1/500.
ICC/IF 1/100 - 1/250.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. The use of an HRP/AP polymerized secondary antibody is recommended.
WB 1/1000 - 1/10000. Predicted molecular weight: 88 kDa.

Application notes

Is unsuitable for IP.

Relevance

Interferon (IFN) Inducible 16 (IFI16) protein belongs to a family of HIN 200 human and mouse proteins. IFI16 is a nuclear protein comtaining regulatory domains such as DNA binding domain, transcriptional regulatory domain and DAPIN/PAAD domain. IFI16 has three isotypes A, B, and C (85-95 kDa), which arise as a result of mRNA alternative splicing. All are phosphorylated on serine and threonine residues and can homo and heterodimerize. Expression is restricted to the nuclei of hematopoietic cells, fibroblasts and epithelial cells. IFI16 expression in hematopoietic cells of myeloid lineage is tightly regulated and highly induced in the differentiation and proliferation of the cell. Due to its localization in the nucleus, regulation of protein expression, and ability to bind DNA, it is assumed that IFI16 has a role in transcription regulation of cell differentiation. In addition, it was found that IFI16 can act as a transcriptional repressor and is involved in regulation and activation of p53 in cancer cells.
Cellular localization

Nucleus. Cytoplasm. Note: Cellular distribution is dependent on the acetylation status of the multipartite nuclear localization signal (NLS); NLS acetylation promotes cytoplasmic localization.
Database links
- Entrez Gene: 3428 Human
- Omim: 147586 Human
- SwissProt: Q16666 Human
Alternative names
- IFI16 antibody
- Gamma interferon inducible protein 16 antibody
- Gamma-interferon-inducible protein Ifi16 antibody
- IF16_HUMAN antibody
- Ifi204 antibody
- IFNGIP1 antibody
- interferon activated gene 204 antibody
- Interferon gamma induced protein IFI 16 antibody
- Interferon gamma inducible protein 16 antibody
- Interferon-inducible myeloid differentiation transcriptional activator antibody
- p204 antibody
- PYHIN2 antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] (ab169788)

ab169788 showing -ve staining in Human gastric adenocarcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-IFI16 antibody [EPR11767(B)] (ab169788)

All lanes : Anti-IFI16 antibody [EPR11767(B)] (ab169788) at 1/1000 dilution

Lane 1 : U937 treated with IFN gamma cell lysate
Lane 2 : U937 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : Raji cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Standard HRP labeled goat anti-rabbit at 1/2000 dilution

Developed using the ECL technique.

Predicted band size: 88 kDa
Immunocytochemistry/ Immunofluorescence - Anti-IFI16 antibody [EPR11767(B)] (ab169788)

Immunofluorescence analysis of Raji cells labeling IFI16, using ab169788 at a 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-IFI16 antibody [EPR11767(B)] (ab169788)

Immunocytochemistry/Immunofluorescence analysis of Jurkat (human acute T cell leukemia) cells labelling IFI16 with ab169788 at 1/100. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).

The image shows nuclear staining on Jurkat cells.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] (ab169788)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling IFI16, using ab169788 at a 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] (ab169788)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human bone marrow tissue labeling IFI16, using ab169788 at a 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] (ab169788)

ab169788 showing +ve staining in Human normal spleen tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] (ab169788)

ab169788 showing -ve staining in Human normal uterus tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cytometry (Intracellular) - Anti-IFI16 antibody [EPR11767(B)] (ab169788)

Intracellular Flow Cytometry analysis of Raji (human Burkitt's lymphoma) cells labelling IFI16 (red) with ab169788 at dilution of 1/500. The secondary antibody used was Alexa Fluor^® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde. Isotype control antibody was (ab172730) Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.
Flow Cytometry (Intracellular) - Anti-IFI16 antibody [EPR11767(B)] (ab169788)

Intracellular Flow Cytometry analysis of Jurkat (human acute T cell leukemia) cells labelling IFI16 (red) with ab169788 at dilution of 1/500. The secondary antibody used was Alexa Fluor^® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde. Isotype control antibody was (ab172730) Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.
Anti-IFI16 antibody [EPR11767(B)] (ab169788)

Flow cytometry protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

Datasheet download

Download

Publishing research using ab169788? Please let us know so that we can cite the reference in this datasheet.

ab169788 has been referenced in 2 publications.

He Q et al. IFI16 promotes human embryonic stem cell trilineage specification through interaction with p53. NPJ Regen Med 5:18 (2020). PubMed: 33298947
Wiley CD et al. Secretion of leukotrienes by senescent lung fibroblasts promotes pulmonary fibrosis. JCI Insight 4:N/A (2019). PubMed: 31687975

Customer reviews and Q&As

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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Isotype control

Positive Controls

Recombinant Protein

Applications

The Abpromise guarantee

Target

Relevance

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

Datasheet download

References (2)

Customer reviews and Q&As