RecombinantRabMAb

Recombinant Anti-IFI16 antibody [EPR11767(B)] (ab169788)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] (ab169788)
  • Western blot - Anti-IFI16 antibody [EPR11767(B)] (ab169788)
  • Immunocytochemistry/ Immunofluorescence - Anti-IFI16 antibody [EPR11767(B)] (ab169788)
  • Immunocytochemistry/ Immunofluorescence - Anti-IFI16 antibody [EPR11767(B)] (ab169788)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] (ab169788)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] (ab169788)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] (ab169788)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] (ab169788)
  • Flow Cytometry - Anti-IFI16 antibody [EPR11767(B)] (ab169788)
  • Flow Cytometry - Anti-IFI16 antibody [EPR11767(B)] (ab169788)
  • Anti-IFI16 antibody [EPR11767(B)] (ab169788)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11767(B)] to IFI16
  • Suitable for: ICC/IF, IHC-P, Flow Cyt, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-IFI16 antibody [EPR11767(B)]
    See all IFI16 primary antibodies

  • Description

    Rabbit monoclonal [EPR11767(B)] to IFI16

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IHC-P, Flow Cyt, WBmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide corresponding to residues in Human IFI16 (UniProt ID: Q16666).

  • Positive control

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab169788 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/100 - 1/250.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. The use of an HRP/AP polymerized secondary antibody is recommended.
Flow Cyt
1/500.
WB
1/1000 - 1/10000. Predicted molecular weight: 88 kDa.
Notes
ICC/IF
1/100 - 1/250.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. The use of an HRP/AP polymerized secondary antibody is recommended.
Flow Cyt
1/500.
WB
1/1000 - 1/10000. Predicted molecular weight: 88 kDa.
  • Application notes
    Is unsuitable for IP.

    • Target

    • Relevance

      Interferon (IFN) Inducible 16 (IFI16) protein belongs to a family of HIN 200 human and mouse proteins. IFI16 is a nuclear protein comtaining regulatory domains such as DNA binding domain, transcriptional regulatory domain and DAPIN/PAAD domain. IFI16 has three isotypes A, B, and C (85-95 kDa), which arise as a result of mRNA alternative splicing. All are phosphorylated on serine and threonine residues and can homo and heterodimerize. Expression is restricted to the nuclei of hematopoietic cells, fibroblasts and epithelial cells. IFI16 expression in hematopoietic cells of myeloid lineage is tightly regulated and highly induced in the differentiation and proliferation of the cell. Due to its localization in the nucleus, regulation of protein expression, and ability to bind DNA, it is assumed that IFI16 has a role in transcription regulation of cell differentiation. In addition, it was found that IFI16 can act as a transcriptional repressor and is involved in regulation and activation of p53 in cancer cells.

    • Cellular localization

      Nucleus. Cytoplasm. Note: Cellular distribution is dependent on the acetylation status of the multipartite nuclear localization signal (NLS); NLS acetylation promotes cytoplasmic localization.

    • Database links

      • Alternative names

        • IFI16 antibody
        • Gamma interferon inducible protein 16 antibody
        • Gamma-interferon-inducible protein Ifi16 antibody
        • IF16_HUMAN antibody
        • Ifi204 antibody
        • IFNGIP1 antibody
        • interferon activated gene 204 antibody
        • Interferon gamma induced protein IFI 16 antibody
        • Interferon gamma inducible protein 16 antibody
        • Interferon-inducible myeloid differentiation transcriptional activator antibody
        • p204 antibody
        • PYHIN2 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] (ab169788)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] (ab169788)

        ab169788 showing -ve staining in Human gastric adenocarcinoma tissue.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Western blot - Anti-IFI16 antibody [EPR11767(B)] (ab169788)
        Western blot - Anti-IFI16 antibody [EPR11767(B)] (ab169788)
        All lanes : Anti-IFI16 antibody [EPR11767(B)] (ab169788) at 1/1000 dilution

        Lane 1 : U937 treated with IFN gamma cell lysate
        Lane 2 : U937 cell lysate
        Lane 3 : Jurkat cell lysate
        Lane 4 : Raji cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Standard HRP labeled goat anti-rabbit at 1/2000 dilution

        Developed using the ECL technique.

        Predicted band size: 88 kDa

      • Immunocytochemistry/ Immunofluorescence - Anti-IFI16 antibody [EPR11767(B)] (ab169788)
        Immunocytochemistry/ Immunofluorescence - Anti-IFI16 antibody [EPR11767(B)] (ab169788)
        Immunofluorescence analysis of Raji cells labeling IFI16, using ab169788 at a 1/100 dilution.
      • Immunocytochemistry/ Immunofluorescence - Anti-IFI16 antibody [EPR11767(B)] (ab169788)
        Immunocytochemistry/ Immunofluorescence - Anti-IFI16 antibody [EPR11767(B)] (ab169788)

        Immunocytochemistry/Immunofluorescence analysis of Jurkat (human acute T cell leukemia) cells labelling IFI16 with ab169788 at 1/100. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).

        The image shows nuclear staining on Jurkat cells.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] (ab169788)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] (ab169788)

        Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling IFI16, using ab169788 at a 1/100 dilution.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] (ab169788)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] (ab169788)

        Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human bone marrow tissue labeling IFI16, using ab169788 at a 1/100 dilution.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] (ab169788)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] (ab169788)

        ab169788 showing +ve staining in Human normal spleen tissue.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] (ab169788)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] (ab169788)

        ab169788 showing -ve staining in Human normal uterus tissue.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Flow Cytometry - Anti-IFI16 antibody [EPR11767(B)] (ab169788)
        Flow Cytometry - Anti-IFI16 antibody [EPR11767(B)] (ab169788)

        Flow cytometry analysis of Raji (human Burkitt's lymphoma) cells labelling IFI16 (red) with ab169788 at dilution of 1/500. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde. Isotype control antibody was (ab172730) Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.

      • Flow Cytometry - Anti-IFI16 antibody [EPR11767(B)] (ab169788)
        Flow Cytometry - Anti-IFI16 antibody [EPR11767(B)] (ab169788)

        Flow cytometry analysis of Jurkat (human acute T cell leukemia) cells labelling IFI16 (red) with ab169788 at dilution of 1/500. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde. Isotype control antibody was (ab172730) Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.

      • Anti-IFI16 antibody [EPR11767(B)] (ab169788)
        Anti-IFI16 antibody [EPR11767(B)] (ab169788)

      References (1)

      Publishing research using ab169788? Please let us know so that we can cite the reference in this datasheet.

      ab169788 has been referenced in 1 publication.

      • Wiley CD  et al. Secretion of leukotrienes by senescent lung fibroblasts promotes pulmonary fibrosis. JCI Insight 4:N/A (2019). PubMed: 31687975

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