Anti-IFI16 antibody [EPR11767(B)] Recombinant (ab169788)

Product name

Anti-IFI16 antibody [EPR11767(B)]
See all IFI16 primary antibodies
Description

Rabbit monoclonal [EPR11767(B)] to IFI16
Host species

Rabbit
Tested applications

Suitable for: ICC/IF, IHC-P, Flow Cyt, WBmore details
Unsuitable for: IP
Species reactivity

Reacts with: Human
Immunogen

Synthetic peptide corresponding to residues in Human IFI16 (UniProt ID: Q16666).
Positive control
- Purchase matching WB positive control:Recombinant Human IFI16 protein
- Recombinant Human IFI16 protein (ab158724) can be used as a positive control in WB. Human tonsil and bone marrow tissues. Raji cells. IFN gamma-treated U937, U937, Jurkat and Raji cell lysates.
General notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents.
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR11767(B)
Isotype

IgG
Research areas

Positive Controls

Our Abpromise guarantee covers the use of ab169788 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
ICC/IF		1/100 - 1/250.
IHC-P		1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. The use of an HRP/AP polymerized secondary antibody is recommended.
Flow Cyt		1/500.
WB		1/1000 - 1/10000. Predicted molecular weight: 88 kDa.

Application notes

Is unsuitable for IP.

Relevance

Interferon (IFN) Inducible 16 (IFI16) protein belongs to a family of HIN 200 human and mouse proteins. IFI16 is a nuclear protein comtaining regulatory domains such as DNA binding domain, transcriptional regulatory domain and DAPIN/PAAD domain. IFI16 has three isotypes A, B, and C (85-95 kDa), which arise as a result of mRNA alternative splicing. All are phosphorylated on serine and threonine residues and can homo and heterodimerize. Expression is restricted to the nuclei of hematopoietic cells, fibroblasts and epithelial cells. IFI16 expression in hematopoietic cells of myeloid lineage is tightly regulated and highly induced in the differentiation and proliferation of the cell. Due to its localization in the nucleus, regulation of protein expression, and ability to bind DNA, it is assumed that IFI16 has a role in transcription regulation of cell differentiation. In addition, it was found that IFI16 can act as a transcriptional repressor and is involved in regulation and activation of p53 in cancer cells.
Cellular localization

Nucleus. Cytoplasm. Note: Cellular distribution is dependent on the acetylation status of the multipartite nuclear localization signal (NLS); NLS acetylation promotes cytoplasmic localization.
Database links
- Entrez Gene: 3428 Human
- Omim: 147586 Human
- SwissProt: Q16666 Human
- Unigene: 380250 Human
Alternative names
- IFI16 antibody
- Gamma interferon inducible protein 16 antibody
- Gamma-interferon-inducible protein Ifi16 antibody
- IF16_HUMAN antibody
- Ifi204 antibody
- IFNGIP1 antibody
- interferon activated gene 204 antibody
- Interferon gamma induced protein IFI 16 antibody
- Interferon gamma inducible protein 16 antibody
- Interferon-inducible myeloid differentiation transcriptional activator antibody
- p204 antibody
- PYHIN2 antibody
see all

Western blot - Anti-IFI16 antibody [EPR11767(B)] (ab169788)

All lanes : Anti-IFI16 antibody [EPR11767(B)] (ab169788) at 1/1000 dilution

Lane 1 : U397 treated with IFN gamma cell lysate
Lane 2 : U397 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : Raji cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Standard HRP labeled goat anti-rabbit at 1/2000 dilution

Developed using the ECL technique.

Predicted band size: 88 kDa
Immunocytochemistry/ Immunofluorescence - Anti-IFI16 antibody [EPR11767(B)] (ab169788)

Immunofluorescence analysis of Raji cells labeling IFI16, using ab169788 at a 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-IFI16 antibody [EPR11767(B)] (ab169788)

Immunocytochemistry/Immunofluorescence analysis of Jurkat (human acute T cell leukemia) cells labelling IFI16 with ab169788 at 1/100. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).

The image shows nuclear staining on Jurkat cells.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] (ab169788)

ab169788 showing -ve staining in Human gastric adenocarcinoma tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] (ab169788)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling IFI16, using ab169788 at a 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] (ab169788)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human bone marrow tissue labeling IFI16, using ab169788 at a 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] (ab169788)

ab169788 showing +ve staining in Human normal spleen tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFI16 antibody [EPR11767(B)] (ab169788)

ab169788 showing -ve staining in Human normal uterus tissue.
Flow Cytometry - Anti-IFI16 antibody [EPR11767(B)] (ab169788)

Flow cytometry analysis of Raji (human Burkitt's lymphoma) cells labelling IFI16 (red) with ab169788 at dilution of 1/500. The secondary antibody used was Alexa Fluor^® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde. Isotype control antibody was (ab172730) Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.
Flow Cytometry - Anti-IFI16 antibody [EPR11767(B)] (ab169788)

Flow cytometry analysis of Jurkat (human acute T cell leukemia) cells labelling IFI16 (red) with ab169788 at dilution of 1/500. The secondary antibody used was Alexa Fluor^® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde. Isotype control antibody was (ab172730) Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.

Datasheet

Flow cytometry protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

ab169788 has not yet been referenced specifically in any publications.

Publishing research using ab169788? Please let us know so that we can cite the reference in this datasheet.

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Anti-IFI16 antibody [EPR11767(B)] (ab169788)

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