Key features and details
- Rabbit polyclonal to IFI35
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human, Pig
- Isotype: IgG
Product nameAnti-IFI35 antibody
See all IFI35 primary antibodies
DescriptionRabbit polyclonal to IFI35
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human, Pig
Recombinant fragment (His-T7-tag) corresponding to Human IFI35 aa 2-286. Two N-terminal tags. Expressed in E.coli.
SAPLDAALHALQEEQARLKMRLWDLQQLRKELGDSPKDKVPFSVPKIPLV FRGHTQQDPEVPKSLVSNLRIHCPLLAGSALITFDDPKVAEQVLQQKEHT INMEECRLRVQVQPLELPMVTTIQMSSQLSGRRVLVTGFPASLRLSEEEL LDKLEIFFGKTRNGGGDVDVRELLPGSVMLGFARDGVAQRLCQIGQFTVP LGGQQVPLRVSPYVNGEIQKAEIRSQPVPRSVLVLNIPDILDGPELHDVL EIHFQKPTRGGGEVEALTVVPQGQQGLAVFTSESG
Database link: P80217
- WB: Recombinant human IFI35 protein; Human lung lysate; HeLa whole cell lysate (ab150035); Pig liver lysate. IHC-P: Human liver tissue. ICC/IF: HeLa cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab233415 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 - 20 µg/ml.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 0.2 - 2 µg/ml.|
FunctionNot yet known.
Tissue specificityIn a wide range of cell types, including fibroblasts, macrophages, and epithelial cells.
Sequence similaritiesBelongs to the NMI family.
Cellular localizationNucleus. Nuclear following IFN treatment.
- Information by UniProt
- FLJ21753 antibody
- IFI 35 antibody
- Ifi-35 antibody
Anti-IFI35 antibody (ab233415) at 2 µg/ml + Recombinant human IFI35 protein
HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
All lanes : Anti-IFI35 antibody (ab233415) at 2 µg/ml
Lane 1 : Human lung lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : Pig liver lysate
All lanes : HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Formalin-fixed, paraffin-embedded human liver tissue stained for IFI35 using ab233415 at 10 μg/ml in immunohistochemical analysis. DAB staining.
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for IFI35 (green) using ab233415 at 20 μg/ml in ICC/IF. FITC staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233415 has not yet been referenced specifically in any publications.