Key features and details
- Rabbit polyclonal to IFIT1
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-IFIT1 antibody
See all IFIT1 primary antibodies
DescriptionRabbit polyclonal to IFIT1
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment corresponding to Human IFIT1 aa 1-181.
Database link: P09914
- WB: HeLa whole cell lysate (ab150035). Rat and mouse liver tissue lysates. ICC/IF: HepG2 cells. IHC-P: Human pancreatic cancer and liver cancer tissues.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol, PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab236256 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/200.|
|IHC-P||1/20 - 1/200.|
|WB||1/200 - 1/5000. Predicted molecular weight: 55 kDa.|
Sequence similaritiesBelongs to the IFIT family.
Contains 10 TPR repeats.
- Information by UniProt
- C56 antibody
- G10P1 antibody
- GARG 16 antibody
All lanes : Anti-IFIT1 antibody (ab236256) at 1/200 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : Rat liver tissue lysate
Lane 3 : Mouse liver tissue lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 55 kDa
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for IFIT1 using ab236256 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
Paraffin-embedded human pancreatic cancer tissue stained for IFIT1 using ab236256 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human liver cancer tissue stained for IFIT1 using ab236256 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236256 has not yet been referenced specifically in any publications.