Overview

  • Product name

    Anti-IFIT1 antibody [OTI3G8]
    See all IFIT1 primary antibodies
  • Description

    Mouse monoclonal [OTI3G8] to IFIT1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human IFIT1. Recombinant full length Human IFIT1 produced in HEK293T cells (NP_000899).
    Database link: P09914

  • Positive control

    • WB: HEK293T cell lysate transfected with pCMV6-ENTRY IFIT1 cDNA. IHC-P: Human liver carcinoma, Human ovary adenocarcinoma, kidney and liver tissues. ICC/IF: HT29 cells and COS7 cells transiently transfected by pCMV6-ENTRY IFIT1 cDNA. Flow: HEK293T cells transfected with pCMV6-ENTRY IFIT1 overexpress plasmid
  • General notes

    Stable for 12 months from date of receipt.

    The clone number has been updated from 3G8 to OTI3G8, both clone numbers name the same clone.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 48% PBS, 1% BSA
  • Concentration information loading...
  • Purity

    IgG fraction
  • Purification notes

    ab118062 is purified from TCS by affinity chromatography.
  • Clonality

    Monoclonal
  • Clone number

    OTI3G8
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab118062 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 55 kDa.
IHC-P 1/50.
Flow Cyt 1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/50 - 1/100.

Target

  • Sequence similarities

    Belongs to the IFIT family.
    Contains 10 TPR repeats.
  • Information by UniProt
  • Database links

  • Alternative names

    • C56 antibody
    • G10P1 antibody
    • GARG 16 antibody
    • GARG16 antibody
    • IFI 56 antibody
    • IFI 56K antibody
    • IFI-56K antibody
    • IFI56 antibody
    • IFIT-1 antibody
    • Ifit1 antibody
    • IFIT1_HUMAN antibody
    • IFNAI1 antibody
    • Interferon alpha inducible protein (MW 56kD) antibody
    • Interferon induced 56 kDa protein antibody
    • Interferon induced protein 56 antibody
    • Interferon inducible mRNA 561 antibody
    • Interferon-induced 56 kDa protein antibody
    • Interferon-induced protein with tetratricopeptide repeats 1 antibody
    • ISG56 antibody
    • OTTHUMP00000020062 antibody
    • P56 antibody
    • RNM561 antibody
    see all

Images

  • All lanes : Anti-IFIT1 antibody [OTI3G8] (ab118062) at 1/500 dilution

    Lane 1 : HEK293T cell lyaste, transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK293T cell lysate, transfected with pCMV6-ENTRY IFIT1 cDNA

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 55 kDa



    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC203320)
  • ab118062 at 1/50 dilution staining IFIT1 in Paraffin-embedded Human Kidney tissue by Immunohistochemistry.
  • ab118062 at 1/50 dilution staining IFIT1 in Paraffin-embedded Human Liver tissue by Immunohistochemistry.
  • ab118062 at 1/50 dilution staining IFIT1 in Paraffin-embedded Human Liver carcinoma tissue by Immunohistochemistry.
  • ab118062 at 1/50 dilution staining IFIT1 in Paraffin-embedded Human Ovary adenocarcinoma tissue by Immunohistochemistry.
  • ab118062 at 1/50 dilution staining IFIT1 in COS7 cells transiently transfected by pCMV6-ENTRY IFIT1 by Immunofluorescence.
  • ab118062 at 1/50 dilution staining IFIT1 in HT29 cells by Immunofluorescence.
  • ab118062 at 1/100 dilution staining IFIT1 in HEK293T cells transfected with either pCMV6-ENTRY IFIT1 overexpress plasmid(Red) or empty vector control plasmid(Blue) by Flow Cytometry.

References

ab118062 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Question
Answer

I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by passing it on to your purchasing department. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. If you have questions on how to use the credit note, please contact our accounting department.

Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up