Anti-IFIT1 antibody [OTI3G8] (ab118062)
Key features and details
- Mouse monoclonal [OTI3G8] to IFIT1
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Human, African green monkey
- Isotype: IgG1
Overview
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Product name
Anti-IFIT1 antibody [OTI3G8]
See all IFIT1 primary antibodies -
Description
Mouse monoclonal [OTI3G8] to IFIT1 -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human, African green monkey -
Immunogen
Recombinant full length protein corresponding to Human IFIT1. Recombinant full length Human IFIT1 produced in HEK293T cells (NP_000899).
Database link: P09914 -
Positive control
- WB: HEK293T cell lysate transfected with pCMV6-ENTRY IFIT1 cDNA. IHC-P: Human liver carcinoma, Human ovary adenocarcinoma, kidney and liver tissues. ICC/IF: HT29 cells and COS7 cells transiently transfected by pCMV6-ENTRY IFIT1 cDNA. Flow Cyt (Intra): HEK293T cells transfected with pCMV6-ENTRY IFIT1 overexpress plasmid
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General notes
The clone number has been updated from 3G8 to OTI3G8, both clone numbers name the same clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. The product can be stored for up to 12 months. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 48% PBS, 1% BSA -
Concentration information loading...
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Purity
IgG fraction -
Purification notes
ab118062 is purified from TCS by affinity chromatography. -
Clonality
Monoclonal -
Clone number
OTI3G8 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab118062 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/100.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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WB |
1/500 - 1/1000. Predicted molecular weight: 55 kDa.
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IHC-P |
1/50.
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ICC/IF |
1/50 - 1/100.
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Notes |
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Flow Cyt (Intra)
1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
WB
1/500 - 1/1000. Predicted molecular weight: 55 kDa. |
IHC-P
1/50. |
ICC/IF
1/50 - 1/100. |
Target
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Sequence similarities
Belongs to the IFIT family.
Contains 10 TPR repeats. - Information by UniProt
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Database links
- Entrez Gene: 3434 Human
- Omim: 147690 Human
- SwissProt: P09914 Human
- Unigene: 20315 Human
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Alternative names
- C56 antibody
- G10P1 antibody
- GARG 16 antibody
see all
Images
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All lanes : Anti-IFIT1 antibody [OTI3G8] (ab118062) at 1/500 dilution
Lane 1 : HEK293T cell lyaste, transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK293T cell lysate, transfected with pCMV6-ENTRY IFIT1 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 55 kDa
HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC203320) -
ab118062 at 1/50 dilution staining IFIT1 in Paraffin-embedded Human Kidney tissue by Immunohistochemistry.
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ab118062 at 1/50 dilution staining IFIT1 in Paraffin-embedded Human Liver tissue by Immunohistochemistry.
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ab118062 at 1/50 dilution staining IFIT1 in Paraffin-embedded Human Liver carcinoma tissue by Immunohistochemistry.
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ab118062 at 1/50 dilution staining IFIT1 in Paraffin-embedded Human Ovary adenocarcinoma tissue by Immunohistochemistry.
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ab118062 at 1/50 dilution staining IFIT1 in COS7 cells transiently transfected by pCMV6-ENTRY IFIT1 by Immunofluorescence.
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ab118062 at 1/50 dilution staining IFIT1 in HT29 cells by Immunofluorescence.
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ab118062 at 1/100 dilution staining IFIT1 in HEK293T cells transfected with either pCMV6-ENTRY IFIT1 overexpress plasmid(Red) or empty vector control plasmid(Blue) by Flow Cytometry (Intracellular).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab118062 has been referenced in 2 publications.
- Gao Y et al. Fusobacterium nucleatum stimulates cell proliferation and promotes PD-L1 expression via IFIT1-related signal in colorectal cancer. Neoplasia 35:100850 (2023). PubMed: 36371909
- Berard AR et al. Comparative proteomic analyses of two reovirus T3D subtypes and comparison to T1L identifies multiple novel proteins in key cellular pathogenic pathways. Proteomics 15:2113-35 (2015). PubMed: 25900405