Key features and details
- Mouse monoclonal [OTI1G1] to IFIT3/P60
- Suitable for: WB, Flow Cyt, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-IFIT3/P60 antibody [OTI1G1]
See all IFIT3/P60 primary antibodies
DescriptionMouse monoclonal [OTI1G1] to IFIT3/P60
Tested applicationsSuitable for: WB, Flow Cyt, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee
Recombinant full length protein corresponding to Human IFIT3/P60 aa 1-490. Produced in HEK-293T cells (NP_001540).
MSEVTKNSLEKILPQLKCHFTWNLFKEDSVSRDLEDRVCNQIEFLNTEFK ATMYNLLAYIKHLDGNNEAALECLRQAEELIQQEHADQAEIRSLVTWGNY AWVYYHLGRLSDAQIYVDKVKQTCKKFSNPYSIEYSELDCEEGWTQLKCG RNERAKVCFEKALEEKPNNPEFSSGLAIAMYHLDNHPEKQFSTDVLKQAI ELSPDNQYVKVLLGLKLQKMNKEAEGEQFVEEALEKSPCQTDVLRSAAKF YRRKGDLDKAIELFQRVLESTPNNGYLYHQIGCCYKAKVRQMQNTGESEA SGNKEMIEALKQYAMDYSNKALEKGLNPLNAYSDLAEFLETECYQTPFNK EVPDAEKQQSHQRYCNLQKYNGKSEDTAVQHGLEGLSISKKSTDKEEIKD QPQNVSENLLPQNAPNYWYLQGLIHKQNGDLLQAAKCYEKELGRLLRDAP SGIGSIFLSASELEDGSEEMGQGAVSSSPRELLSNSEQLN
Database link: O14879
- WB: HepG2, HeLa, A549, COS-7, Jurkat and MCF7 extracts; HEK-293T cells transfected with pCMV6-ENTRY IFIT3/P60 cDNA. ICC/IF: COS-7 (transiently transfected by pCMV6-ENTRY IFIT3/P60 cDNA) and HT-29 cells. Flow Cyt: Jurkat cells.
Clone OTI1G1 (formerly 1G1).
Protein previously labeled as IFIT3.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 1% BSA
Concentration information loading...
Purification notesPurified from cell culture supernatant by affinity chromatography.
- HeLa whole cell lysate (ab150035)
- HepG2 whole cell lysate (ab166833)
- MCF7 whole cell lysate (ab29537)
- HeLa whole cell lysate (ab29545)
- Jurkat whole cell lysate (ab30128)
- MCF7 whole cell lysate (ab3871)
- Jurkat whole cell lysate (ab7899)
- HepG2 whole cell lysate (ab7900)
- A549 whole cell lysate (ab7910)
Our Abpromise guarantee covers the use of ab118045 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Predicted molecular weight: 56 kDa.|
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Sequence similaritiesBelongs to the IFIT family.
Contains 8 TPR repeats.
- Information by UniProt
- CIG 49 antibody
- CIG49 antibody
- GARG 49 antibody
All lanes : Anti-IFIT3/P60 antibody [OTI1G1] (ab118045) at 1/200 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) extracts
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) extracts
Lane 3 : SVT2 extracts
Lane 4 : A549 (Human lung carcinoma cell line) extracts
Lane 5 : COS-7 (African green monkey kidney fibroblast-like cell line) extracts
Lane 6 : Jurkat (Human T cell leukemia cell line from peripheral blood) extracts
Lane 7 : MDCK (Canine kidney cell line) extracts
Lane 8 : PC-12 (Rat adrenal gland pheochromocytoma cell line) extracts
Lane 9 : MCF7 (Human breast adenocarcinoma cell line) extracts
Lysates/proteins at 35 µg per lane.
Predicted band size: 56 kDa
pCMV6-ENTRY IFIT3/P60 cDNA transfected COS-7 (African green monkey kidney fibroblast-like cell line) cells stained for IFIT3/P60 using ab118045 at a 1/100 dilution in ICC/IF.
ab118045 at 1/100 dilution staining IFIT3/P60 in Jurkat (Human T cell leukemia cell line from peripheral blood) cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).
HT-29 (Human colorectal adenocarcinoma cell line) cells stained for IFIT3/P60 using ab118045 at a 1/100 dilution in ICC/IF.
All lanes : Anti-IFIT3/P60 antibody [OTI1G1] (ab118045) at 1/2000 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells, transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK-293T cells, transfected with pCMV6-ENTRY IFIT3/P60 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 56 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab118045 has not yet been referenced specifically in any publications.