Product nameAnti-IFIT3/P60 antibody [OTI1G1]
See all IFIT3/P60 primary antibodies
DescriptionMouse monoclonal [OTI1G1] to IFIT3/P60
Tested applicationsSuitable for: WB, Flow Cyt, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee
Recombinant full length protein corresponding to Human IFIT3/P60 aa 1-490. Produced in HEK-293T cells (NP_001540).
MSEVTKNSLEKILPQLKCHFTWNLFKEDSVSRDLEDRVCNQIEFLNTEFK ATMYNLLAYIKHLDGNNEAALECLRQAEELIQQEHADQAEIRSLVTWGNY AWVYYHLGRLSDAQIYVDKVKQTCKKFSNPYSIEYSELDCEEGWTQLKCG RNERAKVCFEKALEEKPNNPEFSSGLAIAMYHLDNHPEKQFSTDVLKQAI ELSPDNQYVKVLLGLKLQKMNKEAEGEQFVEEALEKSPCQTDVLRSAAKF YRRKGDLDKAIELFQRVLESTPNNGYLYHQIGCCYKAKVRQMQNTGESEA SGNKEMIEALKQYAMDYSNKALEKGLNPLNAYSDLAEFLETECYQTPFNK EVPDAEKQQSHQRYCNLQKYNGKSEDTAVQHGLEGLSISKKSTDKEEIKD QPQNVSENLLPQNAPNYWYLQGLIHKQNGDLLQAAKCYEKELGRLLRDAP SGIGSIFLSASELEDGSEEMGQGAVSSSPRELLSNSEQLN
Database link: O14879
- WB: HepG2, HeLa, A549, COS-7, Jurkat and MCF7 extracts; HEK-293T cells transfected with pCMV6-ENTRY IFIT3/P60 cDNA. ICC/IF: COS-7 (transiently transfected by pCMV6-ENTRY IFIT3/P60 cDNA) and HT-29 cells. FC: Jurkat cells.
Clone OTI1G1 (formerly 1G1).
Protein previously labeled as IFIT3.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 1% BSA
Concentration information loading...
Purification notesPurified from cell culture supernatant by affinity chromatography.
- HeLa whole cell lysate (ab150035)
- HepG2 whole cell lysate (ab166833)
- MCF7 whole cell lysate (ab29537)
- HeLa whole cell lysate (ab29545)
- Jurkat whole cell lysate (ab30128)
- MCF7 whole cell lysate (ab3871)
- Jurkat whole cell lysate (ab7899)
- HepG2 whole cell lysate (ab7900)
- A549 whole cell lysate (ab7910)
Our Abpromise guarantee covers the use of ab118045 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Predicted molecular weight: 56 kDa.|
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Sequence similaritiesBelongs to the IFIT family.
Contains 8 TPR repeats.
- Information by UniProt
- CIG 49 antibody
- CIG49 antibody
- GARG 49 antibody
All lanes : Anti-IFIT3/P60 antibody [OTI1G1] (ab118045) at 1/200 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) extracts
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) extracts
Lane 3 : SVT2 extracts
Lane 4 : A549 (Human lung carcinoma cell line) extracts
Lane 5 : COS-7 (African green monkey kidney fibroblast-like cell line) extracts
Lane 6 : Jurkat (Human T cell leukemia cell line from peripheral blood) extracts
Lane 7 : MDCK (Canine kidney cell line) extracts
Lane 8 : PC-12 (Rat adrenal gland pheochromocytoma cell line) extracts
Lane 9 : MCF7 (Human breast adenocarcinoma cell line) extracts
Lysates/proteins at 35 µg per lane.
Predicted band size: 56 kDa
pCMV6-ENTRY IFIT3/P60 cDNA transfected COS-7 (African green monkey kidney fibroblast-like cell line) cells stained for IFIT3/P60 using ab118045 at a 1/100 dilution in ICC/IF.
ab118045 at 1/100 dilution staining IFIT3/P60 in Jurkat (Human T cell leukemia cell line from peripheral blood) cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).
HT-29 (Human colorectal adenocarcinoma cell line) cells stained for IFIT3/P60 using ab118045 at a 1/100 dilution in ICC/IF.
All lanes : Anti-IFIT3/P60 antibody [OTI1G1] (ab118045) at 1/2000 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells, transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK-293T cells, transfected with pCMV6-ENTRY IFIT3/P60 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 56 kDa
ab118045 has not yet been referenced specifically in any publications.