Overview

  • Product name

  • Description

    Rabbit polyclonal to IFITM1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human IFITM1 aa 1-36.
    Sequence:

    MHKEEHEVAVLGAPPSTILPRSTVINIHSETSVPDH


    Database link: P13164

  • Positive control

    • IHC-P: Human epididymis. WB: CAPAN-2 cell lysate. ICC/IF: A431 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab224063 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100

WB 1/100 - 1/250. Predicted molecular weight: 13 kDa.
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    IFN-induced antiviral protein that mediate cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus, and dengue virus by inhibiting the early step(s) of replication. Plays a key role in the antiproliferative action of IFN-gamma either by inhibiting the ERK activition or by arresting cell growth in G1 phase in a p53-dependent manner. Implicated in the control of cell growth. Component of a multimeric complex involved in the transduction of antiproliferative and homotypic adhesion signals.
  • Sequence similarities

    Belongs to the CD225 family.
  • Post-translational
    modifications

    Palmitoylation on membrane-proximal cysteines controls clustering in membrane compartments and antiviral activity against influenza virus.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 9-27 antibody
    • CD 225 antibody
    • CD 225 antigen antibody
    • CD225 antibody
    • CD225 antigen antibody
    • Dispanin subfamily A member 2a antibody
    • DSPA2a antibody
    • IFI 17 antibody
    • IFI17 antibody
    • IFITM1 antibody
    • IFM1_HUMAN antibody
    • Interferon induced protein 17 antibody
    • interferon induced transmembrane protein 1 (9-27) antibody
    • Interferon induced transmembrane protein 1 antibody
    • Interferon inducible protein 9-27 antibody
    • Interferon-induced protein 17 antibody
    • Interferon-induced transmembrane protein 1 antibody
    • Interferon-inducible protein 9-27 antibody
    • Leu 13 antibody
    • Leu 13 antigen antibody
    • Leu-13 antigen antibody
    • LEU13 antibody
    see all

Images

  • Anti-IFITM1 antibody (ab224063) at 1/100 dilution + CAPAN-2 cell lysate

    Predicted band size: 13 kDa

  • PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for IFITM1 (green) using ab224063 (4 µg/ml) in ICC/IF.

  • Paraffin embedded human epididymis tissue stained for IFITM1 with ab224063 (1/200 dilution) in immunohistochemical analysis.

References

This product has been referenced in:

  • Yang J  et al. Combination of IFITM1 knockdown and radiotherapy inhibits the growth of oral cancer. Cancer Sci 109:3115-3128 (2018). Read more (PubMed: 29770536) »
See 1 Publication for this product

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