Recombinant
RabMAb

Recombinant Anti-IFITM1 antibody [EPR22620-12] (ab233545)

Overview

  • Product name

    Anti-IFITM1 antibody [EPR22620-12]
    See all IFITM1 primary antibodies
  • Description

    Rabbit monoclonal [EPR22620-12] to IFITM1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IF, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human IFITM1 aa 1-100. The exact sequence is proprietary.
    Database link: P13164

  • Positive control

    • WB: K562, TF-1 and HeLa whole cell lysates. IP: K562 whole cell lysate. IHC-P: Human cerebrum and tonsil tissue. ICC/IF: K562 cells. Flow cyt: K562 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab233545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 17 kDa (predicted molecular weight: 13 kDa).
Flow Cyt 1/500.
ICC/IF 1/500.
IP 1/30.
IHC-P 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    IFN-induced antiviral protein that mediate cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus, and dengue virus by inhibiting the early step(s) of replication. Plays a key role in the antiproliferative action of IFN-gamma either by inhibiting the ERK activition or by arresting cell growth in G1 phase in a p53-dependent manner. Implicated in the control of cell growth. Component of a multimeric complex involved in the transduction of antiproliferative and homotypic adhesion signals.
  • Sequence similarities

    Belongs to the CD225 family.
  • Post-translational
    modifications

    Palmitoylation on membrane-proximal cysteines controls clustering in membrane compartments and antiviral activity against influenza virus.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 9-27 antibody
    • CD 225 antibody
    • CD 225 antigen antibody
    • CD225 antibody
    • CD225 antigen antibody
    • Dispanin subfamily A member 2a antibody
    • DSPA2a antibody
    • IFI 17 antibody
    • IFI17 antibody
    • IFITM1 antibody
    • IFM1_HUMAN antibody
    • Interferon induced protein 17 antibody
    • interferon induced transmembrane protein 1 (9-27) antibody
    • Interferon induced transmembrane protein 1 antibody
    • Interferon inducible protein 9-27 antibody
    • Interferon-induced protein 17 antibody
    • Interferon-induced transmembrane protein 1 antibody
    • Interferon-inducible protein 9-27 antibody
    • Leu 13 antibody
    • Leu 13 antigen antibody
    • Leu-13 antigen antibody
    • LEU13 antibody
    see all

Images

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed,  0.1% Triton X-100 permeabilized K562 (human chronic myelogenous leukemia cell line from bone marrow) or A549 (human lung carcinoma cell line) cells labeling IFITM1 with ab233545 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in K-562 cells.  Negative control: A549 (PMID: 25105503). The nuclear cpounter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

    PBS only control: Use PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • All lanes : Anti-IFITM1 antibody [EPR22620-12] (ab233545) at 1/2000 dilution

    Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
    Lane 2 : A549 (human lung carcinoma cell line) whole cell lysate
    Lane 3 : Untreated HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 4 : HeLa, treated with 10ng/ml hIFN-a for 16 hours, whole cell lysate
    Lane 5 : TF-1 (human bone marrow erythroleukemia cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 13 kDa
    Observed band size: 17 kDa
    why is the actual band size different from the predicted?


    Exposure time: 8 seconds


    Blocking and dilution buffer: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 25105503) IFN-α induce the expression of IFITM1(PMID: 27852071).

    Negative control: A549 (PMID: 25105503).

     

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling IFITM1  with ab233545 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on lymphocytes of human tonsil is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    The section was incubated with ab233545 for 10 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

     

  • Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-fixed K562 (human chronic myelogenous leukemia cell line from bone marrow) (Right) or A549 (human lung carcinoma cell line) (Left) cells labeling IFITM1 with ab233545 at 1/500 (red) compared with a Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue).

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

    Negative control: A549 (PMID: 25105503).

  • IFITM1 was immunoprecipitated from 0.35 mg of K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate with ab233545 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab233545 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.

    Lane 1: K562 whole cell lysate 10 μg (Input).
    Lane 2: ab233545 IP in K562 whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab233545 in K562 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 3 seconds.

  • Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling IFITM1  with ab233545 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on blood vessels of human cerebrum (PMID: 24603679). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    The section was incubated with ab233545 for 10 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

     

References

ab233545 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab233545.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up