• Product name
    Anti-IFN gamma Receptor beta / AF-1 antibody
    See all IFN gamma Receptor beta / AF-1 primary antibodies
  • Description
    Rabbit polyclonal to IFN gamma Receptor beta / AF-1
  • Host species
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human, Pig
    Predicted to work with: Cow, Baboon, Deer
  • Immunogen

    Synthetic peptide corresponding to Human IFN gamma Receptor beta/ AF-1 aa 250 to the C-terminus conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab90070)

  • Positive control
    • This antibody gave a positive signal in the following lysates: Tissues: Human spinal cord; Mouse liver; Human skeletal muscle; Mouse kidney. Whole cell: Hela nuclear; F9; A431



Our Abpromise guarantee covers the use of ab77246 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 38 kDa).


  • Function
    Part of the receptor for interferon gamma. Required for signal transduction. This accessory factor is an integral part of the IFN-gamma signal transduction pathway and is likely to interact with GAF, JAK1, and/or JAK2.
  • Involvement in disease
    Defects in IFNGR2 are a cause of mendelian susceptibility to mycobacterial disease (MSMD) [MIM:209950]; also known as familial disseminated atypical mycobacterial infection. This rare condition confers predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals. The pathogenic mechanism underlying MSMD is the impairment of interferon-gamma mediated immunity, whose severity determines the clinical outcome. Some patients die of overwhelming mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop, later in life, disseminated but curable infections with tuberculoid granulomas. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance.
  • Sequence similarities
    Belongs to the type II cytokine receptor family.
    Contains 2 fibronectin type-III domains.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • AF 1 antibody
    • AF-1 antibody
    • AF1 antibody
    • IFGR 2 antibody
    • IFGR2 antibody
    • IFN gamma Receptor beta antibody
    • IFN-gamma receptor 2 antibody
    • IFN-gamma-R2 antibody
    • IFNGR 2 antibody
    • IFNGR2 antibody
    • IFNGT 1 antibody
    • IFNGT1 antibody
    • IMD28 antibody
    • INGR2_HUMAN antibody
    • Interferon gamma receptor 2 (interferon gamma transducer 1) antibody
    • Interferon gamma receptor 2 antibody
    • Interferon gamma receptor accessory factor 1 antibody
    • Interferon gamma receptor beta chain antibody
    • Interferon gamma receptor chain 2 antibody
    • Interferon gamma transducer 1 antibody
    see all


  • All lanes : Anti-IFN gamma Receptor beta / AF-1 antibody (ab77246) at 1 µg/ml

    Lane 1 : Human spinal cord tissue lysate - total protein (ab29188)
    Lane 2 : Liver (Mouse) Tissue Lysate
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
    Lane 4 : F9 (Mouse embryonic carcinoma cell line)
    Lane 5 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 6 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 7 : Kidney (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 38 kDa
    Observed band size: 49 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 16 kDa, 48 kDa (possible cleavage fragment), 77 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 2 minutes

    Interferon-gamma receptor beta chain has a predicted molecular weight of 38 kDa, however it is extensively glycosylated and has been shown to run in the 49 kDa region (SwissProt data). The bands present at 48 kDa are representative of the protein minus its 27 amino acid signal peptide. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.


This product has been referenced in:
  • Zhang S  et al. Swiprosin-1 deficiency impairs macrophage immune response of septic mice. JCI Insight 3:N/A (2018). Read more (PubMed: 29415882) »
  • Bi D  et al. Effects of leukotriene B4 on interleukin-32, interferon-? and chemokines in rats with rheumatoid arthritis. Exp Ther Med 14:2925-2930 (2017). Read more (PubMed: 28912850) »

See all 3 Publications for this product

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