Anti-IFN gamma Receptor beta / AF-1 antibody - Conformation-Specific (ab175878)

Overview

  • Product name
    Anti-IFN gamma Receptor beta / AF-1 antibody - Conformation-Specific
    See all IFN gamma Receptor beta / AF-1 primary antibodies
  • Description
    Rabbit polyclonal to IFN gamma Receptor beta / AF-1 - Conformation-Specific
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human IFN gamma Receptor beta/ AF-1 aa 308-337 (C terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: P38484

  • Positive control
    • MDA-MB453 cell lysate; Human lung carcinoma tissue; NCA-H292 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab175878 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 38 kDa.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Part of the receptor for interferon gamma. Required for signal transduction. This accessory factor is an integral part of the IFN-gamma signal transduction pathway and is likely to interact with GAF, JAK1, and/or JAK2.
  • Involvement in disease
    Defects in IFNGR2 are a cause of mendelian susceptibility to mycobacterial disease (MSMD) [MIM:209950]; also known as familial disseminated atypical mycobacterial infection. This rare condition confers predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals. The pathogenic mechanism underlying MSMD is the impairment of interferon-gamma mediated immunity, whose severity determines the clinical outcome. Some patients die of overwhelming mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop, later in life, disseminated but curable infections with tuberculoid granulomas. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance.
  • Sequence similarities
    Belongs to the type II cytokine receptor family.
    Contains 2 fibronectin type-III domains.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AF 1 antibody
    • AF-1 antibody
    • AF1 antibody
    • IFGR 2 antibody
    • IFGR2 antibody
    • IFN gamma Receptor beta antibody
    • IFN-gamma receptor 2 antibody
    • IFN-gamma-R2 antibody
    • IFNGR 2 antibody
    • IFNGR2 antibody
    • IFNGT 1 antibody
    • IFNGT1 antibody
    • IMD28 antibody
    • INGR2_HUMAN antibody
    • Interferon gamma receptor 2 (interferon gamma transducer 1) antibody
    • Interferon gamma receptor 2 antibody
    • Interferon gamma receptor accessory factor 1 antibody
    • Interferon gamma receptor beta chain antibody
    • Interferon gamma receptor chain 2 antibody
    • Interferon gamma transducer 1 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human lung carcinoma tissue labeling IFN gamma Receptor beta with ab175878 at 1/50 dilution followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Anti-IFN gamma Receptor beta / AF-1 antibody - Conformation-Specific (ab175878) at 1/1000 dilution + MDA-MB453 cell lysate at 35 µg

    Predicted band size: 38 kDa

  • Flow cytometric analysis of NCI-H292 cells labeling IFN gamma Receptor beta with ab175878 at 1/10 dilution (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

This product has been referenced in:
  • Xiao F  et al. Sex-dependent aortic valve pathology in patients with rheumatic heart disease. PLoS One 12:e0180230 (2017). IHC-P ; Human . Read more (PubMed: 28662157) »
  • Iskandar R  et al. Expression of pericardial fluid T-cells and related inflammatory cytokines in patients with chronic heart failure. Exp Ther Med 13:1850-1858 (2017). Read more (PubMed: 28565777) »

See all 4 Publications for this product

Customer reviews and Q&As

The IFNGR2 antibody ab175878 is not expected to react with IFNGR1, as the amino acid sequence the antibody was raised against is not found in IFNGR1. However, we do not have experimental evidence of specificity for just IFNGR2.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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