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Synthetic peptide within Human IFN gamma Receptor beta/ AF-1 aa 308-337 (C terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: P38484
Our Abpromise guarantee covers the use of ab175878 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 38 kDa.|
|Flow Cyt||1/10 - 1/50.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human lung carcinoma tissue labeling IFN gamma Receptor beta with ab175878 at 1/50 dilution followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of NCI-H292 cells labeling IFN gamma Receptor beta with ab175878 at 1/10 dilution (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"