Recombinant
RabMAb

Recombinant Anti-IFN gamma Receptor beta/AF-1 antibody [EPR22171] (ab224197)

Overview

  • Product name

    Anti-IFN gamma Receptor beta/AF-1 antibody [EPR22171]
    See all IFN gamma Receptor beta/AF-1 primary antibodies
  • Description

    Rabbit monoclonal [EPR22171] to IFN gamma Receptor beta/AF-1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human IFN gamma Receptor beta/AF-1 aa 1-250. The exact sequence is proprietary.
    Database link: P38484

  • Positive control

    • ICC/IF: HEK-293T cells transfected with a Myc-tagged IFN gamma Receptor beta/AF-1 expression construct. Flow Cyt: HEK-293T cells transfected with a Myc-tagged IFN gamma Receptor beta/AF-1 expression construct.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab224197 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500.
Flow Cyt 1/400.

Target

  • Function

    Part of the receptor for interferon gamma. Required for signal transduction. This accessory factor is an integral part of the IFN-gamma signal transduction pathway and is likely to interact with GAF, JAK1, and/or JAK2.
  • Involvement in disease

    Defects in IFNGR2 are a cause of mendelian susceptibility to mycobacterial disease (MSMD) [MIM:209950]; also known as familial disseminated atypical mycobacterial infection. This rare condition confers predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals. The pathogenic mechanism underlying MSMD is the impairment of interferon-gamma mediated immunity, whose severity determines the clinical outcome. Some patients die of overwhelming mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop, later in life, disseminated but curable infections with tuberculoid granulomas. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance.
  • Sequence similarities

    Belongs to the type II cytokine receptor family.
    Contains 2 fibronectin type-III domains.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • AF 1 antibody
    • AF-1 antibody
    • AF1 antibody
    • IFGR 2 antibody
    • IFGR2 antibody
    • IFN gamma Receptor beta antibody
    • IFN-gamma receptor 2 antibody
    • IFN-gamma-R2 antibody
    • IFNGR 2 antibody
    • IFNGR2 antibody
    • IFNGT 1 antibody
    • IFNGT1 antibody
    • IMD28 antibody
    • INGR2_HUMAN antibody
    • Interferon gamma receptor 2 (interferon gamma transducer 1) antibody
    • Interferon gamma receptor 2 antibody
    • Interferon gamma receptor accessory factor 1 antibody
    • Interferon gamma receptor beta chain antibody
    • Interferon gamma receptor chain 2 antibody
    • Interferon gamma transducer 1 antibody
    see all

Images

  • Flow cytometric analysis of HEK-293T (human embryonic kidney epithelial cell) transfected with a Myc-tagged IFN gamma Receptor beta/AF-1 expression construct cell line labeling IFN gamma Receptor beta/AF-1 with ab224197 at 1/400 dilution.

    Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

    Cells were surface stained with isotype control Rabbit monoclonal IgG (ab172730) (Left panel) or IFN gamma Receptor beta/AF-1 (Right panel), then co-stained with Alexa Fluor® 647 conjugated-Myc-tag antibody.

    Gated on viable cells.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) cells transfected with a Myc-tagged IFN gamma Receptor beta/AF-1 expression construct labeling IFN gamma Receptor beta/AF-1 with ab224197 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing positive staining in HEK-293T cells transfected with a Myc-tagged IFN gamma Receptor beta/AF-1 expression construct. The nuclear counterstain is DAPI (blue).
    Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red) and Myc-Tag (9B11) Mouse mAb (Alexa Fluor® 647 Conjugate) (white).
    The negative control is the secondary antibody only.

References

ab224197 has not yet been referenced specifically in any publications.

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