Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22171] to IFN gamma Receptor beta/AF-1
- Suitable for: ELISA, ICC/IF, Flow Cyt
- Reacts with: Human
Product nameAnti-IFN gamma Receptor beta/AF-1 antibody [EPR22171]
See all IFN gamma Receptor beta/AF-1 primary antibodies
DescriptionRabbit monoclonal [EPR22171] to IFN gamma Receptor beta/AF-1
Tested applicationsSuitable for: ELISA, ICC/IF, Flow Cytmore details
Species reactivityReacts with: Human
Recombinant fragment within Human IFN gamma Receptor beta/AF-1 aa 1-250. The exact sequence is proprietary.
Database link: P38484
- ICC/IF: HEK-293T cells transfected with a Myc-tagged IFN gamma Receptor beta/AF-1 expression construct. Flow Cyt: HEK-293T cells transfected with a Myc-tagged IFN gamma Receptor beta/AF-1 expression construct.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab224197 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
FunctionPart of the receptor for interferon gamma. Required for signal transduction. This accessory factor is an integral part of the IFN-gamma signal transduction pathway and is likely to interact with GAF, JAK1, and/or JAK2.
Involvement in diseaseDefects in IFNGR2 are a cause of mendelian susceptibility to mycobacterial disease (MSMD) [MIM:209950]; also known as familial disseminated atypical mycobacterial infection. This rare condition confers predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals. The pathogenic mechanism underlying MSMD is the impairment of interferon-gamma mediated immunity, whose severity determines the clinical outcome. Some patients die of overwhelming mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop, later in life, disseminated but curable infections with tuberculoid granulomas. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance.
Sequence similaritiesBelongs to the type II cytokine receptor family.
Contains 2 fibronectin type-III domains.
- Information by UniProt
- AF 1 antibody
- AF-1 antibody
- AF1 antibody
Flow cytometric analysis of HEK-293T (human embryonic kidney epithelial cell) transfected with a Myc-tagged IFN gamma Receptor beta/AF-1 expression construct cell line labeling IFN gamma Receptor beta/AF-1 with ab224197 at 1/400 dilution.
Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.
Cells were surface stained with isotype control Rabbit monoclonal IgG (ab172730) (Left panel) or IFN gamma Receptor beta/AF-1 (Right panel), then co-stained with Alexa Fluor® 647 conjugated-Myc-tag antibody.
Gated on viable cells.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) cells transfected with a Myc-tagged IFN gamma Receptor beta/AF-1 expression construct labeling IFN gamma Receptor beta/AF-1 with ab224197 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing positive staining in HEK-293T cells transfected with a Myc-tagged IFN gamma Receptor beta/AF-1 expression construct. The nuclear counterstain is DAPI (blue).
Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red) and Myc-Tag (9B11) Mouse mAb (Alexa Fluor® 647 Conjugate) (white).
The negative control is the secondary antibody only.
ELISA analysis of Human IFNGR2 recombinant protein at 1000 ng/mL with ab224197. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab224197 has not yet been referenced specifically in any publications.