Recombinant Anti-IFN gamma Receptor beta/AF-1 antibody [EPR22171] - BSA and Azide free (ab240559)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22171] to IFN gamma Receptor beta/AF-1 - BSA and Azide free
- Suitable for: ELISA, ICC/IF, Flow Cyt
- Reacts with: Human, Recombinant fragment
Related conjugates and formulations
Overview
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Product name
Anti-IFN gamma Receptor beta/AF-1 antibody [EPR22171] - BSA and Azide free
See all IFN gamma Receptor beta/AF-1 primary antibodies -
Description
Rabbit monoclonal [EPR22171] to IFN gamma Receptor beta/AF-1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ELISA, ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: HEK-293T cells transfected with a Myc-tagged IFN gamma Receptor beta/AF-1 expression construct.
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General notes
ab240559 is the carrier-free version of ab224197.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22171 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab240559 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
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Notes |
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ELISA
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. |
Target
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Function
Part of the receptor for interferon gamma. Required for signal transduction. This accessory factor is an integral part of the IFN-gamma signal transduction pathway and is likely to interact with GAF, JAK1, and/or JAK2. -
Involvement in disease
Defects in IFNGR2 are a cause of mendelian susceptibility to mycobacterial disease (MSMD) [MIM:209950]; also known as familial disseminated atypical mycobacterial infection. This rare condition confers predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals. The pathogenic mechanism underlying MSMD is the impairment of interferon-gamma mediated immunity, whose severity determines the clinical outcome. Some patients die of overwhelming mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop, later in life, disseminated but curable infections with tuberculoid granulomas. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance. -
Sequence similarities
Belongs to the type II cytokine receptor family.
Contains 2 fibronectin type-III domains. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3460 Human
- Omim: 147569 Human
- SwissProt: P38484 Human
- Unigene: 634632 Human
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Alternative names
- AF 1 antibody
- AF-1 antibody
- AF1 antibody
see all
Images
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Flow cytometric analysis of HEK-293T (human embryonic kidney epithelial cell) transfected with a Myc-tagged IFN gamma Receptor beta/AF-1 expression construct cell line labeling IFN gamma Receptor beta/AF-1 with ab224197 at 1/400 dilution.
Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.
Cells were surface stained with isotype control Rabbit monoclonal IgG (ab172730) (Left panel) or IFN gamma receptor beta/AF-1 (Right panel), then co-stained with Alexa Fluor® 647 conjugated-Myc-tag antibody.
Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224197).
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) cells transfected with a Myc-tagged IFN gamma Receptor beta/AF-1 expression construct labeling IFN gamma Receptor beta/AF-1 with ab224197 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing positive staining in HEK-293T cells transfected with a Myc-tagged IFN gamma Receptor beta/AF-1 expression construct. The nuclear counterstain is DAPI (blue).
Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red) and Myc-Tag (9B11) Mouse mAb (Alexa Fluor® 647 Conjugate) (white).
The negative control is the secondary antibody only.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab224197).
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This data was developed using ab224197, the same antibody clone in a different buffer formulation.
ELISA analysis of Human IFNGR2 recombinant protein at 1000 ng/mL with ab224197. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab240559 has not yet been referenced specifically in any publications.