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Synthetic peptide within Human IFNAR1 aa 500-600 (C terminal). The exact sequence is proprietary.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab45172 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 90,130 kDa (predicted molecular weight: 64 kDa). Due to extensive glycosylation of the mature molecule; the 90 kDa species is a minor incompletely processed species.|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Lanes 1 - 2: Merged signal (red and green). Green - ab45172 observed at 100 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab45172 was shown to recognize IFNAR1 in wild-type HeLa cells as signal was lost at the expected MW in IFNAR1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and IFNAR1 knockout samples were subjected to SDS-PAGE. Ab45172 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"