Overview

  • Product name
    Anti-IFNGR1 antibody [EPR7866]
    See all IFNGR1 primary antibodies
  • Description
    Rabbit monoclonal [EPR7866] to IFNGR1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cyt, ICCmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human IFNGR1 aa 450-550 (C terminal). The exact sequence is proprietary.

  • Positive control
    • WB: MCF7, HeLa, 293T and HepG2 cell lysates. IHC-P: Human tonsil tissue. Flow Cyt: HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Applications

Our Abpromise guarantee covers the use of ab134070 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 54 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC 1/100 - 1/250.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Receptor for interferon gamma. Two receptors bind one interferon gamma dimer.
    • Involvement in disease
      Defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease (MSMD) [MIM:209950]; also known as familial disseminated atypical mycobacterial infection. This rare condition confers predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals. The pathogenic mechanism underlying MSMD is the impairment of interferon-gamma mediated immunity whose severity determines the clinical outcome. Some patients die of overwhelming mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop, later in life, disseminated but curable infections with tuberculoid granulomas. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance.
    • Sequence similarities
      Belongs to the type II cytokine receptor family.
      Contains 2 fibronectin type-III domains.
      Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    • Post-translational
      modifications
      Phosphorylated at Ser/Thr residues.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Antiviral Protein Type II antibody
      • Antiviral protein, type 2 antibody
      • AVP type II antibody
      • AVP, type 2 antibody
      • CD 119 antibody
      • CD119 antibody
      • CD119 antigen antibody
      • CDw119 antibody
      • FLJ45734 antibody
      • IFN gamma R alpha antibody
      • IFN gamma R antibody
      • IFN gamma R1 antibody
      • IFN-gamma receptor 1 antibody
      • IFN-gamma-R1 antibody
      • IFNG R1 antibody
      • IFNGR 1 antibody
      • IFNGR antibody
      • IFNGR1 antibody
      • Immune interferon receptor 1 antibody
      • Immune interferon receptor for antibody
      • INGR1_HUMAN antibody
      • Interferon gamma receptor 1 antibody
      • Interferon gamma receptor alpha chain antibody
      • Interferon gamma receptor alpha chain precursor antibody
      see all

    Images

    • Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling IFNGR1 (red) with ab134070 at a 1/1000 dilution. Cells were fixed with 80% methanol and permeabilized with 0.1% Tween-20. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

    • All lanes : Anti-IFNGR1 antibody [EPR7866] (ab134070) at 1/1000 dilution

      Lane 1 : MCF7 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 54 kDa

    • Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling IFNGR1 with ab134070 at 1/100 dilution.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:
    • Sen A  et al. Rotavirus degrades multiple type interferon receptors to inhibit IFN signaling and protects against mortality from endotoxin in suckling mice. J Virol N/A:N/A (2017). Read more (PubMed: 29070687) »
    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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