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  1. Link

    ifngr1-antibody-epr7866-ab134070.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-IFNGR1 antibody [EPR7866] (ab134070)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (2)

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Western blot - Anti-IFNGR1 antibody [EPR7866] (ab134070)
  • Flow Cytometry (Intracellular) - Anti-IFNGR1 antibody [EPR7866] (ab134070)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFNGR1 antibody [EPR7866] (ab134070)
  • Immunocytochemistry/ Immunofluorescence - Anti-IFNGR1 antibody [EPR7866] (ab134070)
  • Western blot - Anti-IFNGR1 antibody [EPR7866] (ab134070)
  • Western blot - Anti-IFNGR1 antibody [EPR7866] (ab134070)
  • OI-RD Scanning - Anti-IFNGR1 antibody [EPR7866] (ab134070)
  • Anti-IFNGR1 antibody [EPR7866] (ab134070)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7866] to IFNGR1
  • Suitable for: WB, IHC-P, Flow Cyt (Intra), ICC/IF
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free

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Overview

  • Product name

    Anti-IFNGR1 antibody [EPR7866]
    See all IFNGR1 primary antibodies
  • Description

    Rabbit monoclonal [EPR7866] to IFNGR1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt (Intra), ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HEK-293T and HepG2 cell lysates. IHC-P: Human tonsil tissue. Flow Cyt (intra): HeLa cells. ICC/IF: MCF7 cells
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 1.20 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR7866
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Immunology
    • Innate Immunity
    • Cytokines
    • Interferons
    • Microbiology
    • Interspecies Interaction
    • Host Virus Interaction
    • Stem Cells
    • Hematopoietic Progenitors
    • Surface Molecules
    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • ssRNA positive strand virus
    • SARS Coronavirus
    • Immunology
    • Immune System Diseases
    • Antiviral Signaling
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • B Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • NK Cell Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Dendritic Cell Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Monocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Neutrophil Lineage
    • Microbiology
    • Interspecies Interaction
    • Disease

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-IFNGR1 antibody [EPR7866] (ab200327)
    • Alexa Fluor® 647 Anti-IFNGR1 antibody [EPR7866] (ab200329)
    • Anti-IFNGR1 antibody [EPR7866] - BSA and Azide free (ab226151)
    • Anti-IFNGR1 antibody [EPR7866] - Low endotoxin, Azide free (ab246696)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human IFNGR1 knockout HeLa cell line (ab265111)
    • Human IFNGR1 knockout HEK-293 cell line (ab269471)
  • KO cell lysates

    • Human IFNGR1 knockout HeLa cell lysate (ab257477)
    • Human IFNGR1 knockout HEK-293 cell lysate (ab269636)
  • Positive Controls

    • MCF7 membrane extract lysate (ab29539)
    • HeLa membrane extract lysate (ab29547)
  • Recombinant Protein

    • Recombinant human IFNGR1 protein (ab182677)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab134070 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 75-90 kDa (predicted molecular weight: 54 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt (Intra)
1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF (1)
1/100.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 75-90 kDa (predicted molecular weight: 54 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt (Intra)
1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF
1/100.
Application notes
Is unsuitable for IP.

Target

  • Function

    Receptor for interferon gamma. Two receptors bind one interferon gamma dimer.
  • Involvement in disease

    Defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease (MSMD) [MIM:209950]; also known as familial disseminated atypical mycobacterial infection. This rare condition confers predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals. The pathogenic mechanism underlying MSMD is the impairment of interferon-gamma mediated immunity whose severity determines the clinical outcome. Some patients die of overwhelming mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop, later in life, disseminated but curable infections with tuberculoid granulomas. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance.
  • Sequence similarities

    Belongs to the type II cytokine receptor family.
    Contains 2 fibronectin type-III domains.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
  • Post-translational
    modifications

    Phosphorylated at Ser/Thr residues.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P15260 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3459 Human
    • Omim: 107470 Human
    • SwissProt: P15260 Human
    • Unigene: 520414 Human
    • Alternative names

      • Antiviral Protein Type II antibody
      • Antiviral protein, type 2 antibody
      • AVP type II antibody
      • AVP, type 2 antibody
      • CD 119 antibody
      • CD119 antibody
      • CD119 antigen antibody
      • CDw119 antibody
      • FLJ45734 antibody
      • IFN gamma R alpha antibody
      • IFN gamma R antibody
      • IFN gamma R1 antibody
      • IFN-gamma receptor 1 antibody
      • IFN-gamma-R1 antibody
      • IFNG R1 antibody
      • IFNGR 1 antibody
      • IFNGR antibody
      • IFNGR1 antibody
      • Immune interferon receptor 1 antibody
      • Immune interferon receptor for antibody
      • INGR1_HUMAN antibody
      • Interferon gamma receptor 1 antibody
      • Interferon gamma receptor alpha chain antibody
      • Interferon gamma receptor alpha chain precursor antibody
      see all

    Images

    • Western blot - Anti-IFNGR1 antibody [EPR7866] (ab134070)
      Western blot - Anti-IFNGR1 antibody [EPR7866] (ab134070)
      All lanes : Anti-IFNGR1 antibody [EPR7866] (ab134070) at 1/1000 dilution

      Lane 1 : Wild-type HEK-293 cell lysate
      Lane 2 : IFNGR1 knockout HEK-293 cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : MCF7 cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 54 kDa
      Observed band size: 60-80 kDa why is the actual band size different from the predicted?



      Lanes 1 - 4: Merged signal (red and green). Green - ab134070 observed at 60-80 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.

      ab134070 was shown to react with IFNGR1 in wild-type HEK-293 cells in western blot with loss of signal observed in IFNGR1 knockout sample. Wild-type and IFNGR1 knockout HEK-293 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab134070 and ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Flow Cytometry (Intracellular) - Anti-IFNGR1 antibody [EPR7866] (ab134070)
      Flow Cytometry (Intracellular) - Anti-IFNGR1 antibody [EPR7866] (ab134070)

      Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling IFNGR1 (red) with ab134070 at a 1/1000 dilution. Cells were fixed with 80% methanol and permeabilized with 0.1% Tween-20. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies. 

       

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFNGR1 antibody [EPR7866] (ab134070)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFNGR1 antibody [EPR7866] (ab134070)

      Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling IFNGR1 with ab134070 at 1/100 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-IFNGR1 antibody [EPR7866] (ab134070)
      Immunocytochemistry/ Immunofluorescence - Anti-IFNGR1 antibody [EPR7866] (ab134070)

      Immunocytochemistry/ Immunofluorescence analysis of MCF7 (human breast adenocarcinoma epithelial cell) cells labeling IFNGR1 with purified ab134070 at 1/100 dilution (10 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL) was used as the secondary antibody only control.

    • Western blot - Anti-IFNGR1 antibody [EPR7866] (ab134070)
      Western blot - Anti-IFNGR1 antibody [EPR7866] (ab134070)
      All lanes : Anti-IFNGR1 antibody [EPR7866] (ab134070) at 1/1000 dilution

      Lane 1 : Wild-type HeLa cell lysate
      Lane 2 : IFNGR1 knockout HeLa cell lysate
      Lane 3 : HepG2 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

      Predicted band size: 54 kDa
      Observed band size: 70-95 kDa why is the actual band size different from the predicted?



      Lanes 1-3: Merged signal (red and green). Green - ab134070 observed at 70-95 kDa. Red - loading control ab8245 observed at 36 kDa.

      ab134070 Anti-IFNGR1 antibody [EPR7866] was shown to specifically react with IFNGR1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265111 (knockout cell lysate ab257477) was used. Wild-type and IFNGR1 knockout samples were subjected to SDS-PAGE. ab134070 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

       

    • Western blot - Anti-IFNGR1 antibody [EPR7866] (ab134070)
      Western blot - Anti-IFNGR1 antibody [EPR7866] (ab134070)
      Anti-IFNGR1 antibody [EPR7866] (ab134070) at 1/1000 dilution + HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size: 54 kDa
      Observed band size: 75-90 kDa why is the actual band size different from the predicted?


      Exposure time: 60 seconds


      Blocking/Diluting buffer and concentration: 5% NFDM/TBST

    • OI-RD Scanning - Anti-IFNGR1 antibody [EPR7866] (ab134070)
      OI-RD Scanning - Anti-IFNGR1 antibody [EPR7866] (ab134070)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-IFNGR1 antibody [EPR7866] (ab134070)
      Anti-IFNGR1 antibody [EPR7866] (ab134070)

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (2)

    Publishing research using ab134070? Please let us know so that we can cite the reference in this datasheet.

    ab134070 has been referenced in 2 publications.

    • Martin V  et al. Met inhibition revokes IFN?-induction of PD-1 ligands in MET-amplified tumours. Br J Cancer 120:527-536 (2019). PubMed: 30723303
    • Sen A  et al. Rotavirus degrades multiple type interferon receptors to inhibit IFN signaling and protects against mortality from endotoxin in suckling mice. J Virol N/A:N/A (2017). PubMed: 29070687

    Customer reviews and Q&As

    Show All Reviews Q&A
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    Immunocytochemistry/ Immunofluorescence abreview for Anti-IFNGR1 antibody [EPR7866]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Horse Cell (Adult Tenocytes)
    Permeabilization
    Yes - 0.1% Triton-X-100
    Specification
    Adult Tenocytes
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Miss. Emily Smith

    Verified customer

    Submitted Nov 30 2020

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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