Recombinant Anti-IFNGR1 antibody [EPR7866] (ab134070)
![Western blot - Anti-IFNGR1 antibody [EPR7866] (ab134070)](https://www.abcam.com/ps/products/134/ab134070/Images/ab134070-412975-anti-ifngr1-antibody-epr7866-western-blot-hek293-wt-hek293-ifngr1-knockout-hepg2-mcf7.jpg "Western blot - Anti-IFNGR1 antibody [EPR7866] (ab134070)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7866] to IFNGR1
- Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-IFNGR1 antibody [EPR7866]
See all IFNGR1 primary antibodies -
Description
Rabbit monoclonal [EPR7866] to IFNGR1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human IFNGR1 aa 450-550 (C terminal). The exact sequence is proprietary.
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Positive control
- WB: HeLa, HEK-293T and HepG2 cell lysates. IHC-P: Human tonsil tissue. Flow Cyt: HeLa cells. ICC/IF: MCF7 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.20 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7866 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab134070 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 75-90 kDa (predicted molecular weight: 54 kDa). | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| Flow Cyt | 1/1000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| ICC/IF | 1/100. |
Target
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Function
Receptor for interferon gamma. Two receptors bind one interferon gamma dimer. -
Involvement in disease
Defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease (MSMD) [MIM:209950]; also known as familial disseminated atypical mycobacterial infection. This rare condition confers predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals. The pathogenic mechanism underlying MSMD is the impairment of interferon-gamma mediated immunity whose severity determines the clinical outcome. Some patients die of overwhelming mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop, later in life, disseminated but curable infections with tuberculoid granulomas. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance. -
Sequence similarities
Belongs to the type II cytokine receptor family.
Contains 2 fibronectin type-III domains.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains. -
Post-translational
modificationsPhosphorylated at Ser/Thr residues. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3459 Human
- Omim: 107470 Human
- SwissProt: P15260 Human
- Unigene: 520414 Human
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Alternative names
- Antiviral Protein Type II antibody
- Antiviral protein, type 2 antibody
- AVP type II antibody
see all
Images
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Western blot - Anti-IFNGR1 antibody [EPR7866] (ab134070)All lanes : Anti-IFNGR1 antibody [EPR7866] (ab134070) at 1/1000 dilution
Lane 1 : Wild-type HEK-293 cell lysate
Lane 2 : IFNGR1 knockout HEK-293 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : MCF7 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 54 kDa
Observed band size: 60-80 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab134070 observed at 60-80 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.
ab134070 was shown to react with IFNGR1 in wild-type HEK-293 cells in western blot with loss of signal observed in IFNGR1 knockout sample. Wild-type and IFNGR1 knockout HEK-293 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab134070 and ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Flow Cytometry - Anti-IFNGR1 antibody [EPR7866] (ab134070)](https://www.abcam.com/ps/products/134/ab134070/Images/ab134070-280386-anti-ifngr1-antibody-epr7866-flow-cytometry.jpg)
Flow Cytometry - Anti-IFNGR1 antibody [EPR7866] (ab134070)Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling IFNGR1 (red) with ab134070 at a 1/1000 dilution. Cells were fixed with 80% methanol and permeabilized with 0.1% Tween-20. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFNGR1 antibody [EPR7866] (ab134070)](https://www.abcam.com/ps/products/134/ab134070/Images/ab134070-148202-anti-ifngr1-antibody-epr7866-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFNGR1 antibody [EPR7866] (ab134070)Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling IFNGR1 with ab134070 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-IFNGR1 antibody [EPR7866] (ab134070)](https://www.abcam.com/ps/products/134/ab134070/Images/ab134070-6-anti-ifngr1-antibody-epr7866-immunocytochemistry-mcf7-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-IFNGR1 antibody [EPR7866] (ab134070)Immunocytochemistry/ Immunofluorescence analysis of MCF7 (human breast adenocarcinoma epithelial cell) cells labeling IFNGR1 with purified ab134070 at 1/100 dilution (10 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL) was used as the secondary antibody only control.
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![Western blot - Anti-IFNGR1 antibody [EPR7866] (ab134070)](https://www.abcam.com/ps/products/134/ab134070/Images/ab134070-391308-anti-ifngr1-antibody-epr7866-western-blot-human-knockout-lysate.jpg)
Western blot - Anti-IFNGR1 antibody [EPR7866] (ab134070)All lanes : Anti-IFNGR1 antibody [EPR7866] (ab134070) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : IFNGR1 knockout HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 54 kDa
Observed band size: 70-95 kDa why is the actual band size different from the predicted?Lanes 1-3: Merged signal (red and green). Green - ab134070 observed at 70-95 kDa. Red - loading control ab8245 observed at 36 kDa.
ab134070 Anti-IFNGR1 antibody [EPR7866] was shown to specifically react with IFNGR1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265111 (knockout cell lysate ab257477) was used. Wild-type and IFNGR1 knockout samples were subjected to SDS-PAGE. ab134070 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-IFNGR1 antibody [EPR7866] (ab134070)](https://www.abcam.com/ps/products/134/ab134070/Images/ab134070-347869-anti-ifngr1-antibody-EPR7866-western-blot-heLa-human.JPG)
Western blot - Anti-IFNGR1 antibody [EPR7866] (ab134070)Anti-IFNGR1 antibody [EPR7866] (ab134070) at 1/1000 dilution + HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 54 kDa
Observed band size: 75-90 kDa why is the actual band size different from the predicted?
Exposure time: 60 secondsBlocking/Diluting buffer and concentration: 5% NFDM/TBST
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![OI-RD Scanning - Anti-IFNGR1 antibody [EPR7866] (ab134070)](https://www.abcam.com/ps/products/134/ab134070/Images/ab134070-148201-anti-ifngr1-antibody-epr7866.jpg)
OI-RD Scanning - Anti-IFNGR1 antibody [EPR7866] (ab134070) -
![Anti-IFNGR1 antibody [EPR7866] (ab134070)](https://www.abcam.com/ps/products/134/ab134070/Images/ab134070-10-benefits-of-recombinant-antibodies.png)
Anti-IFNGR1 antibody [EPR7866] (ab134070)
Protocols
Datasheets and documents
References (2)
ab134070 has been referenced in 2 publications.
- Martin V et al. Met inhibition revokes IFN?-induction of PD-1 ligands in MET-amplified tumours. Br J Cancer 120:527-536 (2019). PubMed: 30723303
- Sen A et al. Rotavirus degrades multiple type interferon receptors to inhibit IFN signaling and protects against mortality from endotoxin in suckling mice. J Virol N/A:N/A (2017). PubMed: 29070687
