Recombinant
RabMAb

Recombinant Anti-IFNGR1 antibody [EPR7866] - BSA and Azide free (ab226151)

Overview

  • Product name

    Anti-IFNGR1 antibody [EPR7866] - BSA and Azide free
    See all IFNGR1 primary antibodies
  • Description

    Rabbit monoclonal [EPR7866] to IFNGR1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues at the C-terminus of Human IFNGR1.

  • Positive control

    • WB: MCF7, HeLa, 293T and HepG2 cell lysates. IHC-P: Human tonsil tissue. Flow Cyt: HeLa cells.
  • General notes

    The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®). 

    ab226151 is a PBS-only buffer formulated version of ab134070, containing no BSA or sodium azide, ideal for antibody labeling. Please refer to ab134070 for information on protocols, dilutions, and image data.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Applications

Our Abpromise guarantee covers the use of ab226151 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 90 kDa (predicted molecular weight: 54 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG (Low endotoxin, Azide free), is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Receptor for interferon gamma. Two receptors bind one interferon gamma dimer.
    • Involvement in disease

      Defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease (MSMD) [MIM:209950]; also known as familial disseminated atypical mycobacterial infection. This rare condition confers predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals. The pathogenic mechanism underlying MSMD is the impairment of interferon-gamma mediated immunity whose severity determines the clinical outcome. Some patients die of overwhelming mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop, later in life, disseminated but curable infections with tuberculoid granulomas. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance.
    • Sequence similarities

      Belongs to the type II cytokine receptor family.
      Contains 2 fibronectin type-III domains.
      Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    • Post-translational
      modifications

      Phosphorylated at Ser/Thr residues.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • Antiviral Protein Type II antibody
      • Antiviral protein, type 2 antibody
      • AVP type II antibody
      • AVP, type 2 antibody
      • CD 119 antibody
      • CD119 antibody
      • CD119 antigen antibody
      • CDw119 antibody
      • FLJ45734 antibody
      • IFN gamma R alpha antibody
      • IFN gamma R antibody
      • IFN gamma R1 antibody
      • IFN-gamma receptor 1 antibody
      • IFN-gamma-R1 antibody
      • IFNG R1 antibody
      • IFNGR 1 antibody
      • IFNGR antibody
      • IFNGR1 antibody
      • Immune interferon receptor 1 antibody
      • Immune interferon receptor for antibody
      • INGR1_HUMAN antibody
      • Interferon gamma receptor 1 antibody
      • Interferon gamma receptor alpha chain antibody
      • Interferon gamma receptor alpha chain precursor antibody
      see all

    Images

    • This Flow Cyt data was generated using the same anti-IFNGR1 antibody clone, EPR7866, in a different buffer formulation (cat# ab134070).

      Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling IFNGR1 (red) with ab134070 at a 1/1000 dilution. Cells were fixed with 80% methanol and permeabilized with 0.1% Tween-20. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134070).

    • This IHC data was generated using the same anti-IFNGR1 antibody clone, EPR7866, in a different buffer formulation (cat# ab134070).

      Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling IFNGR1 with ab134070 at 1/100 dilution.

    References

    ab226151 has not yet been referenced specifically in any publications.

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