Key features and details
- Rabbit polyclonal to IFNGR1 (phospho Y457)
- Suitable for: WB, IHC-P, ELISA
- Reacts with: Human
- Isotype: IgG
Product nameAnti-IFNGR1 (phospho Y457) antibody
See all IFNGR1 primary antibodies
DescriptionRabbit polyclonal to IFNGR1 (phospho Y457)
Specificityab61062 detects endogenous levels of IFNGR1 only when phosphorylated at tyrosine 457.
Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic phosphopeptide derived from human IFNGR1 around the phosphorylation site of tyrosine 457 (GYpDKP).
- Human brain tissue, COS7 cell extract.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab61062 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab61062 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 54 kDa).|
|IHC-P||1/50 - 1/100.|
FunctionReceptor for interferon gamma. Two receptors bind one interferon gamma dimer.
Involvement in diseaseDefects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease (MSMD) [MIM:209950]; also known as familial disseminated atypical mycobacterial infection. This rare condition confers predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals. The pathogenic mechanism underlying MSMD is the impairment of interferon-gamma mediated immunity whose severity determines the clinical outcome. Some patients die of overwhelming mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop, later in life, disseminated but curable infections with tuberculoid granulomas. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance.
Sequence similaritiesBelongs to the type II cytokine receptor family.
Contains 2 fibronectin type-III domains.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
modificationsPhosphorylated at Ser/Thr residues.
- Information by UniProt
- Antiviral Protein Type II antibody
- Antiviral protein, type 2 antibody
- AVP type II antibody
Immunohistochemistry analysis of paraffin-embedded human brain tissue using IFNGR1 (phospho Y457) antibody (ab61062) at 1/50 - 1/100 dilution, in the presence (right panel) or absence (left panel) of phosphopeptide.
All lanes : Anti-IFNGR1 (phospho Y457) antibody (ab61062) at 1/500 dilution
Lane 1 : COS7 cell extract with no phosphopeptide
Lane 2 : COS7 cell extract with phosphopeptide
Predicted band size: 54 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
ab61062 has been referenced in 1 publication.
- Shi J et al. JAK2 V617F stimulates proliferation of erythropoietin-dependent erythroid progenitors and delays their differentiation by activating Stat1 and other nonerythroid signaling pathways. Exp Hematol 44:1044-1058.e5 (2016). PubMed: 27473563