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Rabbit polyclonal to IFNW1
Predicted to work with:
Synthetic peptide corresponding to Human IFNW1 aa 50-150 conjugated to Keyhole Limpet Haemocyanin (KLH).
Database link: P05000
This antibody gave a positive signal in Human Skeletal Muscle tissue lysate.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide Constituent: PBS Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 22 kDa).
Belongs to the alpha/beta interferon family.
Information by UniProt
Interferon alpha II 1 antibody
Western blot - Anti-IFNW1 antibody (ab166645)
All lanes :
Anti-IFNW1 antibody (ab166645) at 1 µg/ml
All lanes :
Human skeletal muscle tissue lysate - total protein (
Lysates/proteins at 20 µg per lane.
Secondary All lanes :
Goat Anti-Rabbit IgG H&L (HRP) (
) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size:
Observed band size:
30 kDa (
why is the actual band size different from the predicted?
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab166645 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution
has not yet been referenced specifically in any publications.
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