Key features and details
- Rabbit polyclonal to IFNW1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-IFNW1 antibody
DescriptionRabbit polyclonal to IFNW1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla
Synthetic peptide corresponding to Human IFNW1 aa 50-150 conjugated to keyhole limpet haemocyanin.
Database link: P05000
- This antibody gave a positive signal in Human Skeletal Muscle tissue lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab166645 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 22 kDa).|
Sequence similaritiesBelongs to the alpha/beta interferon family.
- Information by UniProt
- IFNW1 antibody
- IFNW1_HUMAN antibody
- Interferon alpha II 1 antibody
All lanes : Anti-IFNW1 antibody (ab166645) at 1 µg/ml
All lanes : Human skeletal muscle tissue lysate - total protein (ab29330)
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab166645 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406
ab166645 has not yet been referenced specifically in any publications.