Key features and details
- Rabbit polyclonal to IFRD1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-IFRD1 antibody
See all IFRD1 primary antibodies
DescriptionRabbit polyclonal to IFRD1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to Human IFRD1 aa 1-240.
Database link: O00458
- WB: HeLa and Jurkat whole cell lysate (ab7899); mouse skeletal muscle tissue lysate. IHC-P: Human brain and adrenal gland tissues.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229720 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 50 kDa (predicted molecular weight: 50, 45 kDa).|
|IHC-P||1/20 - 1/200.|
FunctionCould play a role in regulating gene activity in the proliferative and/or differentiative pathways induced by NGF. May be an autocrine factor that attenuates or amplifies the initial ligand-induced signal.
Tissue specificityExpressed in a variety of tissues.
Sequence similaritiesBelongs to the IFRD family.
- Information by UniProt
- 12 O tetradecanoylphorbol 13 acetate induced sequence 7 antibody
- IFRD1 antibody
- IFRD1_HUMAN antibody
All lanes : Anti-IFRD1 antibody (ab229720) at 1/1000 dilution
Lane 1 : Mouse skeletal muscle tissue lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 50, 45 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Paraffin-embedded human brain tissue stained for IFRD1 using ab229720 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human adrenal gland tissue stained for IFRD1 using ab229720 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229720 has not yet been referenced specifically in any publications.