Overview

  • Product name

    Anti-IFT122 antibody
  • Description

    Rabbit polyclonal to IFT122
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 667-883 of Human IFT122 (AAH03045).

  • Positive control

    • 293T whole cell lysate; Hepatoma tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab111838 is purified by antigen-affinity chromatography.
  • Clonality

    Polyclonal
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab111838 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 129 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. or Tris-EDTA buffer (pH 8.0)

Target

  • Function

    Required for cilia formation and Shh signaling during neuronal patterning.
  • Tissue specificity

    Expressed in many tissues. Predominant expression in testis and pituitary.
  • Involvement in disease

    Defects in IFT122 are a cause of cranioectodermal dysplasia type 1 (CED1) [MIM:218330]. CED1 is a disorder characterized by craniofacial, skeletal and ectodermal abnormalities. Clinical features include dolichocephaly (with or without sagittal suture synostosis), scaphocephaly, short stature, limb shortening, short ribs, narrow chest, brachydactyly, renal failure and hepatic fibrosis, small and abnormally shaped teeth, sparse hair, skin laxity and abnormal nails.
  • Sequence similarities

    Contains 7 WD repeats.
  • Cellular localization

    Cytoplasm. Cell projection > cilium. Cytoplasm > cytoskeleton > cilium basal body. Localizes to photoreceptor connecting cilia.
  • Information by UniProt
  • Database links

  • Alternative names

    • CED antibody
    • IF122_HUMAN antibody
    • IFT122 antibody
    • Intraflagellar transport 122 homolog (Chlamydomonas) antibody
    • Intraflagellar transport protein 122 homolog antibody
    • SPG antibody
    • WD repeat domain 10 antibody
    • WD repeat-containing protein 10 antibody
    • WD repeat-containing protein 140 antibody
    • WDR10 antibody
    • WDR10p antibody
    • WDR140 antibody
    see all

Images

  • Anti-IFT122 antibody (ab111838) at 1/1000 dilution + 293T cell line lysate at 30 µg

    Predicted band size: 129 kDa



    7.5% SDS PAGE
  • ab111838, at 1/500 dilution, staining IFT122 in Formalin-fixed, Paraffin-embedded Hepatoma tissue by Immunohistochemistry.

References

ab111838 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab111838.
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