Key features and details
- Rabbit polyclonal to IFT140
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-IFT140 antibody
DescriptionRabbit polyclonal to IFT140
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human IFT140 aa 1291-1462.
QVEIDEYQNYDKAHGALTEAYKCLAKAKAKSPLDQETRLAQLQSRMALVK RFIQARRTYTEDPKESIKQCELLLEEPDLDSTIRIGDVYGFLVEHYVRKE EYQTAYRFLEEMRRRLPLANMSYYVSPQAVDAVHRGLGLPLPRTVPEQVR HNSMEDARELDEEVVEEADDDP
Database link: Q96RY7-1
- IHC-P: Human adrenal gland tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity greater than 95%.
Our Abpromise guarantee covers the use of ab238109 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/500.|
RelevanceIFT140 contains 9 TPR repeats and 5 WD repeats. The function of the IFT140 protein remains unknown.
- Gs114 antibody
- Intraflagellar transport 140 homolog (Chlamydomonas) antibody
- WD and tetratricopeptide repeats protein 2 antibody
- WDTC2 antibody
Paraffin-embedded human adrenal gland tissue stained for IFT140 with ab238109 at a 1/300 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab238109 has not yet been referenced specifically in any publications.