Overview

  • Product name

    Anti-IFT20 antibody
  • Description

    Rabbit polyclonal to IFT20
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human IFT20 (internal sequence). The exact sequence is proprietary.
    Database link: Q8IY31

  • Positive control

    • ICC/IF: HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: PBS, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab228951 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.

Target

  • Relevance

    Eukaryotic cilia are assembled via intraflagellar transport (IFT) in which large protein particles are motored along ciliary microtubules. The IFT20 subunit of a particle is localized to the Golgi complex, plus the basal body and cilia. In living cells, fluorescently tagged IFT20 is highly dynamic and moves between the Golgi complex and the cilium as well as along ciliary microtubules. It has been suggested that IFT20 functions in the delivery of ciliary membrane proteins from the Golgi complex to the cilium. There are three different isoforms.
  • Cellular localization

    Golgi apparatus. Cilium. Centrosome, centriole.
  • Database links

  • Alternative names

    • hIFT20 antibody
    • Intraflagellar transport 20 homolog (Chlamydomonas) antibody
    • Intraflagellar transport protein 20 like antibody
    • Intraflagellar transport protein IFT20 antibody

Images

  • Ice-cold methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for IFT20 (green) using ab228951 at 1/500 dilution in ICC/IF.

    Nuclear counterstain: Hoechst 33342 (blue).

References

ab228951 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab228951.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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