Overview

  • Product name

  • Description

    Rabbit polyclonal to IFT46
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    antigen sequence: ETDSDSDDDD EEHGAPLEGA YDPADYEHLP VSAEIKELFQ YISRYTPQLI DLDHKLKPFI PDFIPAVGDI DAFLKVPRPD GKPDNLGLLV, corresponding to amino acids 44-133 of Human IFT46.

  • Positive control

    • Human bronchus tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab122422 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Forms part of a complex involved in intraflagellar transport (IFT), the bi-directional movement of particles required for the assembly, maintenance and functioning of primary cilia. May play a role in chondrocyte maturation and skeletogenesis.
  • Sequence similarities

    Belongs to the IFT46 family.
  • Cellular localization

    Cytoplasm > cytoskeleton > cilium basal body. Cell projection > cilium. Expression is concentrated at the cilium basal body but is also detected along the length of the cilium.
  • Information by UniProt
  • Database links

  • Alternative names

    • C11orf2 antibody
    • C11orf60 antibody
    • IFT46 antibody
    • IFT46_HUMAN antibody
    • Intraflagellar transport protein 46 homolog antibody
    see all

Images

  • Immunofluorescent staining of Human cell line U-251 MG shows positivity in cytoplasm. Recommended concentration of ab122422 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122422 at 1/1000 dilution staining IFT46 in Paraffin-embedded Human bronchus tissue by Immunohistochemistry.

References

This product has been referenced in:

  • Shaheen R  et al. Bi-allelic Mutations in FAM149B1 Cause Abnormal Primary Cilium and a Range of Ciliopathy Phenotypes in Humans. Am J Hum Genet 104:731-737 (2019). Read more (PubMed: 30905400) »
  • Anderson AE  et al. Tgif1 and Tgif2 Repress Expression of the RabGAP Evi5l. Mol Cell Biol 37:N/A (2017). Read more (PubMed: 27956704) »
See all 3 Publications for this product

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