Overview

  • Product name

  • Description

    Rabbit polyclonal to IFT46
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length protein corresponding to Human IFT46 aa 1-304.
    Database link: Q9NQC8

  • Positive control

    • WB: Mouse skeletal muscle tissue lysate. IHC-P: Human melanoma cancer and liver tissues.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.4
    Preservative: 0.03% Proclin
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity >95%
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab223036 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/200.
WB 1/1000 - 1/5000. Detects a band of approximately 34 kDa (predicted molecular weight: 34,41 kDa).

Target

  • Function

    Forms part of a complex involved in intraflagellar transport (IFT), the bi-directional movement of particles required for the assembly, maintenance and functioning of primary cilia. May play a role in chondrocyte maturation and skeletogenesis.
  • Sequence similarities

    Belongs to the IFT46 family.
  • Cellular localization

    Cytoplasm > cytoskeleton > cilium basal body. Cell projection > cilium. Expression is concentrated at the cilium basal body but is also detected along the length of the cilium.
  • Information by UniProt
  • Database links

  • Alternative names

    • C11orf2 antibody
    • C11orf60 antibody
    • IFT46 antibody
    • IFT46_HUMAN antibody
    • Intraflagellar transport protein 46 homolog antibody
    see all

Images

  • Anti-IFT46 antibody (ab223036) at 1/1000 dilution + Mouse skeletal muscle tissue lysate

    Secondary
    Goat Anti-Rabbit IgG at 1/10000 dilution

    Predicted band size: 34,41 kDa
    Observed band size: 34 kDa
    why is the actual band size different from the predicted?

  • Paraffin-embedded human liver tissue stained for IFT46 using ab223036 at 1/100 dilution in immunohistochemical analysis.

  • Paraffin-embedded human melanoma cancer tissue stained for IFT46 using ab223036 at 1/100 dilution in immunohistochemical analysis.

References

ab223036 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab223036.
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