Key features and details
- Rabbit polyclonal to IFT80
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, 59% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab126784 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionComponent of the intraflagellar transport (IFT) complex B, which is essential for the development and maintenance of motile and sensory cilia.
Involvement in diseaseDefects in IFT80 are the cause of asphyxiating thoracic dystrophy type 2 (ATD2) [MIM:611263]. An autosomal recessive chondrodysplasia characterized by a severely constricted thoracic cage, short-limbed short stature, and polydactyly. It often leads to death in infancy because of respiratory insufficiency. Retinal degeneration, cystic renal disease and hepatic disease can be present in affected individuals who survive early childhood.
Sequence similaritiesContains 7 WD repeats.
Cellular localizationCytoplasm. Cytoplasm > cytoskeleton > cilium basal body. Cytoplasm > cytoskeleton > cilium axoneme. Basal body and ciliary axoneme.
- Information by UniProt
- ATD2 antibody
- Ift80 antibody
- IFT80_HUMAN antibody
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus and nucleoli. Recommended concentration of ab126784 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab126784, at 1/50 dilution, staining IFT80 in formalin-fixed, paraffin-embedded Human kidney tissue, by Immunohistochemistry.
ab126784 has not yet been referenced specifically in any publications.