Overview

  • Product name
  • Description
    Rabbit polyclonal to IFT80
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Xenopus tropicalis
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 114-362 of Human IFT80 (UniProt ID: Q9P2H3).

  • Positive control
    • NT2D1 whole cell lysate; AdCA tissue.
  • General notes
    Keep as concentrated solution.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 10% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab154933 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 88 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Tris-EDTA buffer (pH 8.0) can also be used.

Target

  • Function
    Component of the intraflagellar transport (IFT) complex B, which is essential for the development and maintenance of motile and sensory cilia.
  • Involvement in disease
    Defects in IFT80 are the cause of asphyxiating thoracic dystrophy type 2 (ATD2) [MIM:611263]. An autosomal recessive chondrodysplasia characterized by a severely constricted thoracic cage, short-limbed short stature, and polydactyly. It often leads to death in infancy because of respiratory insufficiency. Retinal degeneration, cystic renal disease and hepatic disease can be present in affected individuals who survive early childhood.
  • Sequence similarities
    Contains 7 WD repeats.
  • Cellular localization
    Cytoplasm. Cytoplasm > cytoskeleton > cilium basal body. Cytoplasm > cytoskeleton > cilium axoneme. Basal body and ciliary axoneme.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATD2 antibody
    • Ift80 antibody
    • IFT80_HUMAN antibody
    • Intraflagellar transport 80 homolog (Chlamydomonas) antibody
    • Intraflagellar transport protein 80 homolog antibody
    • KIAA1374 antibody
    • WD repeat domain 56 antibody
    • WD repeat-containing protein 56 antibody
    • WDR56 antibody
    see all

Images

  • Anti-IFT80 antibody (ab154933) at 1/500 dilution + NT2D1 whole cell lysate at 30 µg

    Predicted band size: 88 kDa



    5% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human AdCA tissue labeling IFT80 with ab154933 at 1/500 dilution.

References

ab154933 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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