Overview

  • Product name

    Anti-IFT88 antibody [EPR14850] - N-terminal
    See all IFT88 primary antibodies
  • Description

    Rabbit monoclonal [EPR14850] to IFT88 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human IFT88 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: Q13099

  • Positive control

    • Human fetal kidney, HepG2 and Jurkat whole cell lysate (ab7899); HepG2 cells.
  • General notes

     

     

     This product was previously labelled as TTC10

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR14850
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab184566 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/260.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB 1/10000 - 1/50000. Detects a band of approximately 94 kDa (predicted molecular weight: 94 kDa).

Target

  • Function

    Involved in primary cilium biogenesis.
  • Tissue specificity

    Expressed in the heart, brain, liver, lung, kidney, skeletal muscle and pancreas.
  • Sequence similarities

    Contains 12 TPR repeats.
  • Cellular localization

    Cytoplasm > cytoskeleton > centrosome > centriole. Cytoplasm > cytoskeleton > cilium basal body.
  • Information by UniProt
  • Database links

  • Alternative names

    • D13S1056E antibody
    • DAF19 antibody
    • hTg737 antibody
    • Ift88 antibody
    • IFT88_HUMAN antibody
    • Intraflagellar transport 88 homolog antibody
    • Intraflagellar transport protein 88 homolog antibody
    • MGC26259 antibody
    • Polaris homolog antibody
    • Probe hTg737 (polycystic kidney disease, autosomal recessive) antibody
    • Recessive polycystic kidney disease protein Tg737 homolog antibody
    • RP11-172H24.2 antibody
    • Tetratricopeptide repeat domain 10 antibody
    • Tetratricopeptide repeat protein 10 antibody
    • TG737 antibody
    • TPR repeat protein 10 antibody
    • TTC10 antibody
    see all

Images

  • All lanes : Anti-IFT88 antibody [EPR14850] - N-terminal (ab184566) at 1/50000 dilution

    Lane 1 : 293 cell lysate
    Lane 2 : Human fetal kidney lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 94 kDa

  • Flow cytometric analysis of HepG2 cells (2% paraformaldehyde-fixed) labeling IFT88 with ab184566 at 1/260 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

  • All lanes : Anti-IFT88 antibody [EPR14850] - N-terminal (ab184566) at 1/10000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 94 kDa

References

This product has been referenced in:

  • Zhang W  et al. IFT52 mutations destabilize anterograde complex assembly, disrupt ciliogenesis and result in short rib polydactyly syndrome. Hum Mol Genet 25:4012-4020 (2016). Read more (PubMed: 27466190) »
See 1 Publication for this product

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