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  1. Link

    iga-antibody-epr5367-76-ab124716.pdf

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Immunology Immunoglobulins Heavy Chain IgA
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RecombinantRabMAb

Recombinant Anti-IgA antibody [EPR5367-76] (ab124716)

  • Datasheet
  • SDS
Reviews (2) Submit a question References (5)

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Immunoprecipitation - Anti-IgA antibody [EPR5367-76] (ab124716)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgA antibody [EPR5367-76] (ab124716)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgA antibody [EPR5367-76] (ab124716)
  • Western blot - Anti-IgA antibody [EPR5367-76] (ab124716)
  • Anti-IgA antibody [EPR5367-76] (ab124716)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5367-76] to IgA
  • Suitable for: WB, IP, ELISA, IHC-P
  • Reacts with: Human

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Overview

  • Product name

    Anti-IgA antibody [EPR5367-76]
    See all IgA primary antibodies
  • Description

    Rabbit monoclonal [EPR5367-76] to IgA
  • Host species

    Rabbit
  • Specificity

    ab124716 reacts with Heavy chain of IgA. It does not react with either kappa or lambda light chain. It does not react with other Ig.
  • Tested applications

    Suitable for: WB, IP, ELISA, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length native protein (purified) corresponding to Human IgA.

  • Positive control

    • Human tonsil, Human plasma, and Human spleen lysates; Human colon tissue, Human tonsil tissue.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR5367-76
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgA

Associated products

  • Alternative Versions

    • Anti-IgA antibody [EPR5367-76] - BSA and Azide free (ab214003)
    • Alexa Fluor® 488 Anti-IgA antibody [EPR5367-76] (ab223410)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab124716 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
IP
1/10 - 1/100.
ELISA
1/250 - 1/4000.
IHC-P (1)
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
IP
1/10 - 1/100.
ELISA
1/250 - 1/4000.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Relevance

    Human IgA (immunoglobulin A) is a glycosylated protein of 160 kDa and is produced as a monomer or as a J chain linked dimer. Monomeric IgA constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces such as saliva, gastrointestinal secretion, bronchial fluids and milk. Mucosal IgA plays a major role in host defence by neutralising infectious agents at mucosal surfaces. The production is usually local and antigen specific IgA producing B cells can be found in regions under the lamina propria where they mature into dimeric IgA producing plasma cells. IgA deficiency is the most common immunodeficiency that may affect both serum and mucosal produced IgA. OR: The secretory component is a component of immunoglobulin A (IgA) which consists of a portion of the polymeric immunoglobulin receptor. Polymeric IgA binds to the polymeric immunoglobulin receptor on the basolateral surface of epithelial cells and is taken up into the cell via transcytosis. The receptor-IgA complex passes through the cellular compartments before being secreted on the luminal surface of the epithelial cells, still attached to the receptor. Proteolysis of the receptor occurs and the dimeric IgA molecule, along with the secretory component, are free to diffuse throughout the lumen.
  • Cellular localization

    Secreted
  • Database links

    • Entrez Gene: 3494 Human
    • Entrez Gene: 5284 Human
    • Omim: 146900 Human
    • Omim: 147000 Human
    • Omim: 173880 Human
    • SwissProt: P01876 Human
    • SwissProt: P01877 Human
    • Alternative names

      • Hepatocellular carcinoma-associated protein TB6 antibody
      • Ig alpha 1 chain C region antibody
      • Ig alpha 2 chain C region antibody
      • IGHA antibody
      • IGHA1 antibody
      • IGHA2 antibody
      • Immunoglobulin heavy constant alpha 1 antibody
      • Immunoglobulin heavy constant alpha 2 A2m marker antibody
      • Immunoglobulin heavy constant alpha 2 antibody
      • PIgR antibody
      • Poly-Ig receptor antibody
      • polymeric immunoglobulin receptor antibody
      • polymeric immunoglobulin receptor Secretory component antibody
      see all

    Images

    • Immunoprecipitation - Anti-IgA antibody [EPR5367-76] (ab124716)
      Immunoprecipitation - Anti-IgA antibody [EPR5367-76] (ab124716)

      Capture ab 1:60 dilution (1μg in 1mg lysate). Primary ab for WB ab124716 at 1:1000 dilution (0.18μg/ml). Secondary ab Anti-Rabbit IgG (HRP), specific to the non5% NFDM/TBST -reduced form of IgG. Secondary ab concentration 1:1500 dilution. Blocking buffer and concentration 5% NFDM/TBST. Diluting buffer and concentration 5% NFDM/TBST Lane 1 (+) Human plasma Lane 2 (-) Rabbit monoclonal IgG (ab172730) instead of ab124716 in Human plasma



      All lanes : Anti-IgA antibody [EPR5367-76] (ab124716) at 0.18 µg/ml (ab124716 at 1:1000 dilution (0.18µg/ml))

      Lane 1 : Human plasma with NFDM/TBST
      Lane 2 : Rabbit monoclonal IgG (ab172730) instead of ab124716 in Human plasma with NFDM/TBST

      Blocking peptides at 5 % per lane.

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution

      Observed band size: 60 kDa why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgA antibody [EPR5367-76] (ab124716)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgA antibody [EPR5367-76] (ab124716)

      ab124716, at 1/100, staining IgA in paraffin embedded Human colon tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgA antibody [EPR5367-76] (ab124716)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgA antibody [EPR5367-76] (ab124716)

      ab124716, at 1/100, staining IgA in paraffin embedded Human tonsil tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Western blot - Anti-IgA antibody [EPR5367-76] (ab124716)
      Western blot - Anti-IgA antibody [EPR5367-76] (ab124716)
      All lanes : Anti-IgA antibody [EPR5367-76] (ab124716) at 1/1000 dilution

      Lane 1 : Human tonsil lysate
      Lane 2 : Human plasma lysate
      Lane 3 : Human spleen lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 37 kDa

    • Anti-IgA antibody [EPR5367-76] (ab124716)
      Anti-IgA antibody [EPR5367-76] (ab124716)

    Protocols

    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (5)

    Publishing research using ab124716? Please let us know so that we can cite the reference in this datasheet.

    ab124716 has been referenced in 5 publications.

    • Biswas S  et al. IgA transcytosis and antigen recognition govern ovarian cancer immunity. Nature N/A:N/A (2021). PubMed: 33536615
    • Verathamjamras C  et al. Aberrant RL2 O-GlcNAc antibody reactivity against serum-IgA1 of patients with colorectal cancer. Glycoconj J 38:55-65 (2021). PubMed: 33608772
    • Liu H  et al. Small airway immunoglobulin A profile in emphysema-predominant chronic obstructive pulmonary disease. Chin Med J (Engl) 133:1915-1921 (2020). PubMed: 32826454
    • Shulzhenko N  et al. CVID enteropathy is characterized by exceeding low mucosal IgA levels and interferon-driven inflammation possibly related to the presence of a pathobiont. Clin Immunol 197:139-153 (2018). PubMed: 30240602
    • Fahrbach KM  et al. Differential binding of IgG and IgA to mucus of the female reproductive tract. PLoS One 8:e76176 (2013). WB ; Human . PubMed: 24098437

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-IgA antibody [EPR5367-76]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate
    Permeabilization
    No
    Specification
    Liver
    Blocking step
    Serum as blocking agent for 30 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Mar 18 2019

    Flow Cytometry abreview for Anti-IgA antibody [EPR5367-76]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (PBMC)
    Permeabilization
    Yes - 4x Cytofix, 45 min, 4 ¯C
    Gating Strategy
    CD19 positive live cells
    Specification
    PBMC
    Preparation
    Cell harvesting/tissue preparation method: PBMC from whole blood by density gradient (lymphoprep, Fresenius)
    Sample buffer: D-PBS+5 % FCS + 2 mM EDTA
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Clara Lettl

    Verified customer

    Submitted Jan 18 2017

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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