• Product name

    Anti-IgA antibody [EPR5367-76]
    See all IgA primary antibodies
  • Description

    Rabbit monoclonal [EPR5367-76] to IgA
  • Host species

  • Specificity

    ab124716 reacts with Heavy chain of IgA. It does not react with either kappa or lambda light chain. It does not react with other Ig.
  • Tested applications

    Suitable for: WB, IP, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length native protein (purified) corresponding to Human IgA.

  • Positive control

    • Human tonsil, Human plasma, and Human spleen lysates; Human colon tissue, Human tonsil tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.


  • Form

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

  • Clone number

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab124716 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
IP 1/10 - 1/100.
ELISA 1/250 - 1/4000.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.


  • Relevance

    Human IgA (immunoglobulin A) is a glycosylated protein of 160 kDa and is produced as a monomer or as a J chain linked dimer. Monomeric IgA constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces such as saliva, gastrointestinal secretion, bronchial fluids and milk. Mucosal IgA plays a major role in host defence by neutralising infectious agents at mucosal surfaces. The production is usually local and antigen specific IgA producing B cells can be found in regions under the lamina propria where they mature into dimeric IgA producing plasma cells. IgA deficiency is the most common immunodeficiency that may affect both serum and mucosal produced IgA. OR: The secretory component is a component of immunoglobulin A (IgA) which consists of a portion of the polymeric immunoglobulin receptor. Polymeric IgA binds to the polymeric immunoglobulin receptor on the basolateral surface of epithelial cells and is taken up into the cell via transcytosis. The receptor-IgA complex passes through the cellular compartments before being secreted on the luminal surface of the epithelial cells, still attached to the receptor. Proteolysis of the receptor occurs and the dimeric IgA molecule, along with the secretory component, are free to diffuse throughout the lumen.
  • Cellular localization

  • Database links

  • Alternative names

    • Hepatocellular carcinoma-associated protein TB6 antibody
    • Ig alpha 1 chain C region antibody
    • Ig alpha 2 chain C region antibody
    • IGHA antibody
    • IGHA1 antibody
    • IGHA2 antibody
    • Immunoglobulin heavy constant alpha 1 antibody
    • Immunoglobulin heavy constant alpha 2 A2m marker antibody
    • Immunoglobulin heavy constant alpha 2 antibody
    • PIgR antibody
    • Poly-Ig receptor antibody
    • polymeric immunoglobulin receptor antibody
    • polymeric immunoglobulin receptor Secretory component antibody
    see all


  • Capture ab 1:60 dilution (1μg in 1mg lysate). Primary ab for WB ab124716 at 1:1000 dilution (0.18μg/ml). Secondary ab Anti-Rabbit IgG (HRP), specific to the non5% NFDM/TBST -reduced form of IgG. Secondary ab concentration 1:1500 dilution. Blocking buffer and concentration 5% NFDM/TBST. Diluting buffer and concentration 5% NFDM/TBST Lane 1 (+) Human plasma Lane 2 (-) Rabbit monoclonal IgG (ab172730) instead of ab124716 in Human plasma

    All lanes : Anti-IgA antibody [EPR5367-76] (ab124716) at 0.18 µg/ml (ab124716 at 1:1000 dilution (0.18µg/ml))

    Lane 1 : Human plasma with NFDM/TBST
    Lane 2 : Rabbit monoclonal IgG (ab172730) instead of ab124716 in Human plasma with NFDM/TBST

    Blocking peptides at 5 % per lane.

    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution

    Observed band size: 60 kDa
    why is the actual band size different from the predicted?

  • ab124716, at 1/100, staining IgA in paraffin embedded Human colon tissue by Immunohistochemistry.
  • ab124716, at 1/100, staining IgA in paraffin embedded Human tonsil tissue by Immunohistochemistry.
  • All lanes : Anti-IgA antibody [EPR5367-76] (ab124716) at 1/1000 dilution

    Lane 1 : Human tonsil lysate
    Lane 2 : Human plasma lysate
    Lane 3 : Human spleen lysate

    Lysates/proteins at 10 µg per lane.

    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 37 kDa


This product has been referenced in:

  • Fahrbach KM  et al. Differential binding of IgG and IgA to mucus of the female reproductive tract. PLoS One 8:e76176 (2013). WB ; Human . Read more (PubMed: 24098437) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Human Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Blocking step
Serum as blocking agent for 30 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Mar 18 2019

Flow Cytometry
Human Cell (PBMC)
Yes - 4x Cytofix, 45 min, 4 ¯C
Gating Strategy
CD19 positive live cells
Cell harvesting/tissue preparation method: PBMC from whole blood by density gradient (lymphoprep, Fresenius)
Sample buffer: D-PBS+5 % FCS + 2 mM EDTA

Clara Lettl

Verified customer

Submitted Jan 18 2017

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