Recombinant
RabMAb

Recombinant Anti-IgA antibody [EPR5367-76] - BSA and Azide free (ab214003)

Overview

  • Product name

    Anti-IgA antibody [EPR5367-76] - BSA and Azide free
    See all IgA primary antibodies
  • Description

    Rabbit monoclonal [EPR5367-76] to IgA - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ELISA, WB, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length native protein (purified) corresponding to Human IgA.

  • Positive control

    • Human tonsil, Human plasma, and Human spleen lysates; Human colon tissue, Human tonsil tissue.
  • General notes

    ab214003 is the carrier-free version of ab124716 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab214003 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at 4°C (stable for up to 12 months). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR5367-76
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab214003 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 37 kDa.
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Target

  • Relevance

    Human IgA (immunoglobulin A) is a glycosylated protein of 160 kDa and is produced as a monomer or as a J chain linked dimer. Monomeric IgA constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces such as saliva, gastrointestinal secretion, bronchial fluids and milk. Mucosal IgA plays a major role in host defence by neutralising infectious agents at mucosal surfaces. The production is usually local and antigen specific IgA producing B cells can be found in regions under the lamina propria where they mature into dimeric IgA producing plasma cells. IgA deficiency is the most common immunodeficiency that may affect both serum and mucosal produced IgA. OR: The secretory component is a component of immunoglobulin A (IgA) which consists of a portion of the polymeric immunoglobulin receptor. Polymeric IgA binds to the polymeric immunoglobulin receptor on the basolateral surface of epithelial cells and is taken up into the cell via transcytosis. The receptor-IgA complex passes through the cellular compartments before being secreted on the luminal surface of the epithelial cells, still attached to the receptor. Proteolysis of the receptor occurs and the dimeric IgA molecule, along with the secretory component, are free to diffuse throughout the lumen.
  • Cellular localization

    Secreted
  • Database links

  • Alternative names

    • Hepatocellular carcinoma-associated protein TB6 antibody
    • Ig alpha 1 chain C region antibody
    • Ig alpha 2 chain C region antibody
    • IGHA antibody
    • IGHA1 antibody
    • IGHA2 antibody
    • Immunoglobulin heavy constant alpha 1 antibody
    • Immunoglobulin heavy constant alpha 2 A2m marker antibody
    • Immunoglobulin heavy constant alpha 2 antibody
    • PIgR antibody
    • Poly-Ig receptor antibody
    • polymeric immunoglobulin receptor antibody
    • polymeric immunoglobulin receptor Secretory component antibody
    see all

Images

  • Capture ab 1:60 dilution (1μg in 1mg lysate). Primary ab for WB ab124716 at 1:1000 dilution (0.18μg/ml). Secondary ab Anti-Rabbit IgG (HRP), specific to the non5% NFDM/TBST -reduced form of IgG. Secondary ab concentration 1:1500 dilution. Blocking buffer and concentration 5% NFDM/TBST. Diluting buffer and concentration 5% NFDM/TBST Lane 1 (+) Human plasma Lane 2 (-) Rabbit monoclonal IgG (ab172730) instead of ab124716 in Human plasma

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124716).

  • ab124716, at 1/100, staining IgA in paraffin embedded Human colon tissue by Immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124716).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • ab124716, at 1/100, staining IgA in paraffin embedded Human tonsil tissue by Immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124716).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

References

This product has been referenced in:

  • Fahrbach KM  et al. Differential binding of IgG and IgA to mucus of the female reproductive tract. PLoS One 8:e76176 (2013). WB ; Human . Read more (PubMed: 24098437) »
See 1 Publication for this product

Customer reviews and Q&As

Application
CyTOF®
Sample
Human Cell (PBMC)
Specification
PBMC

Abcam user community

Verified customer

Submitted Jun 26 2019

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