RecombinantRabMAb

Recombinant Anti-IgA antibody [EPR5367-76] - BSA and Azide free (ab214003)

Reviews (1) Submit a question References (3)
Immunoprecipitation - Anti-IgA antibody [EPR5367-76] - BSA and Azide free (ab214003)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgA antibody [EPR5367-76] - BSA and Azide free (ab214003)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgA antibody [EPR5367-76] - BSA and Azide free (ab214003)
  • Immunohistochemistry (Frozen sections) - Anti-IgA antibody [EPR5367-76] - BSA and Azide free (ab214003)
  • Anti-IgA antibody [EPR5367-76] - BSA and Azide free (ab214003)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5367-76] to IgA - BSA and Azide free
  • Suitable for: IHC-Fr, IHC-P, ELISA, WB, ICC/IF, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-IgA antibody [EPR5367-76] - BSA and Azide free
    See all IgA primary antibodies

  • Description

    Rabbit monoclonal [EPR5367-76] to IgA - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-Fr, IHC-P, ELISA, WB, ICC/IF, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Full length native protein (purified) corresponding to Human IgA.

  • Positive control

    • Human tonsil, Human plasma, and Human spleen lysates; Human colon tissue, Human tonsil tissue.

  • General notes

    ab214003 is the carrier-free version of ab124716 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab214003 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.20
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR5367-76

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab214003 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ELISA
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 37 kDa.
ICC/IF
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Notes
IHC-Fr
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ELISA
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 37 kDa.
ICC/IF
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.

Target

  • Relevance

    Human IgA (immunoglobulin A) is a glycosylated protein of 160 kDa and is produced as a monomer or as a J chain linked dimer. Monomeric IgA constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces such as saliva, gastrointestinal secretion, bronchial fluids and milk. Mucosal IgA plays a major role in host defence by neutralising infectious agents at mucosal surfaces. The production is usually local and antigen specific IgA producing B cells can be found in regions under the lamina propria where they mature into dimeric IgA producing plasma cells. IgA deficiency is the most common immunodeficiency that may affect both serum and mucosal produced IgA. OR: The secretory component is a component of immunoglobulin A (IgA) which consists of a portion of the polymeric immunoglobulin receptor. Polymeric IgA binds to the polymeric immunoglobulin receptor on the basolateral surface of epithelial cells and is taken up into the cell via transcytosis. The receptor-IgA complex passes through the cellular compartments before being secreted on the luminal surface of the epithelial cells, still attached to the receptor. Proteolysis of the receptor occurs and the dimeric IgA molecule, along with the secretory component, are free to diffuse throughout the lumen.

  • Cellular localization

    Secreted

  • Database links

    • Alternative names

      • Hepatocellular carcinoma-associated protein TB6 antibody
      • Ig alpha 1 chain C region antibody
      • Ig alpha 2 chain C region antibody
      • IGHA antibody
      • IGHA1 antibody
      • IGHA2 antibody
      • Immunoglobulin heavy constant alpha 1 antibody
      • Immunoglobulin heavy constant alpha 2 A2m marker antibody
      • Immunoglobulin heavy constant alpha 2 antibody
      • PIgR antibody
      • Poly-Ig receptor antibody
      • polymeric immunoglobulin receptor antibody
      • polymeric immunoglobulin receptor Secretory component antibody
      see all

    Images

    • Immunoprecipitation - Anti-IgA antibody [EPR5367-76] - BSA and Azide free (ab214003)
      Immunoprecipitation - Anti-IgA antibody [EPR5367-76] - BSA and Azide free (ab214003)

      Capture ab 1:60 dilution (1μg in 1mg lysate). Primary ab for WB ab124716 at 1:1000 dilution (0.18μg/ml). Secondary ab Anti-Rabbit IgG (HRP), specific to the non5% NFDM/TBST -reduced form of IgG. Secondary ab concentration 1:1500 dilution. Blocking buffer and concentration 5% NFDM/TBST. Diluting buffer and concentration 5% NFDM/TBST Lane 1 (+) Human plasma Lane 2 (-) Rabbit monoclonal IgG (ab172730) instead of ab124716 in Human plasma

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124716).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgA antibody [EPR5367-76] - BSA and Azide free (ab214003)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgA antibody [EPR5367-76] - BSA and Azide free (ab214003)

      ab124716, at 1/100, staining IgA in paraffin embedded Human colon tissue by Immunohistochemistry.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124716).

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgA antibody [EPR5367-76] - BSA and Azide free (ab214003)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgA antibody [EPR5367-76] - BSA and Azide free (ab214003)

      ab124716, at 1/100, staining IgA in paraffin embedded Human tonsil tissue by Immunohistochemistry.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124716).

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Frozen sections) - Anti-IgA antibody [EPR5367-76] - BSA and Azide free (ab214003)
      Immunohistochemistry (Frozen sections) - Anti-IgA antibody [EPR5367-76] - BSA and Azide free (ab214003)

      Clone EPR5367-76 (ab214003) has been successfully conjugated by Abcam. This image was generated using Anti-IgA antibody [EPR5367-76] (Alexa Fluor® 488). Please refer to ab223410 for protocol details.

      IHC image of IgA staining in a section of frozen normal human tonsil*.

      The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature.  The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab223410 at 1/5000 dilution (shown in green) and counterstained using ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

    • Anti-IgA antibody [EPR5367-76] - BSA and Azide free (ab214003)
      Anti-IgA antibody [EPR5367-76] - BSA and Azide free (ab214003)

    References (3)

    Publishing research using ab214003? Please let us know so that we can cite the reference in this datasheet.

    ab214003 has been referenced in 3 publications.

    • Norman M  et al. Ultra-Sensitive High-Resolution Profiling of Anti-SARS-CoV-2 Antibodies for Detecting Early Seroconversion in COVID-19 Patients. medRxiv N/A:N/A (2020). PubMed: 32511657
    • Norman M  et al. Ultrasensitive high-resolution profiling of early seroconversion in patients with COVID-19. Nat Biomed Eng N/A:N/A (2020). PubMed: 32948854
    • Fahrbach KM  et al. Differential binding of IgG and IgA to mucus of the female reproductive tract. PLoS One 8:e76176 (2013). WB ; Human . PubMed: 24098437

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review

    Filter by Application

    Filter by Species

    Filter by Ratings

    Mass Cytometry abreview for Anti-IgA antibody [EPR5367-76] - BSA and Azide free

     Good
    Abreviews
    abreview image
    Application
    Mass Cytometry
    Sample
    Human Cell (PBMC)
    Specification
    PBMC
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 26 2019

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com