Recombinant

Recombinant Anti-IgA antibody [RM128] (Biotin) (ab224184)

Overview

  • Product name

    Anti-IgA antibody [RM128] (Biotin)
    See all IgA primary antibodies
  • Description

    Rabbit monoclonal [RM128] to IgA (Biotin)
  • Host species

    Rabbit
  • Conjugation

    Biotin
  • Tested applications

    Suitable for: ELISA, IHC-P, Sandwich ELISAmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length protein corresponding to Human IgA.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 50% Glycerol, PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    ab224184 was purified from an animal origin–free culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    RM128
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab224184 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 0.1 - 1 µg/ml.
Sandwich ELISA Use at an assay dependent concentration.

Use: 50 ng/well – 200 ng/well (for Capture); 0.05 μg/mL – 0.2 μg/mL (for Detection).

Target

  • Relevance

    Human IgA (immunoglobulin A) is a glycosylated protein of 160 kDa and is produced as a monomer or as a J chain linked dimer. Monomeric IgA constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces such as saliva, gastrointestinal secretion, bronchial fluids and milk. Mucosal IgA plays a major role in host defence by neutralising infectious agents at mucosal surfaces. The production is usually local and antigen specific IgA producing B cells can be found in regions under the lamina propria where they mature into dimeric IgA producing plasma cells. IgA deficiency is the most common immunodeficiency that may affect both serum and mucosal produced IgA. OR: The secretory component is a component of immunoglobulin A (IgA) which consists of a portion of the polymeric immunoglobulin receptor. Polymeric IgA binds to the polymeric immunoglobulin receptor on the basolateral surface of epithelial cells and is taken up into the cell via transcytosis. The receptor-IgA complex passes through the cellular compartments before being secreted on the luminal surface of the epithelial cells, still attached to the receptor. Proteolysis of the receptor occurs and the dimeric IgA molecule, along with the secretory component, are free to diffuse throughout the lumen.
  • Cellular localization

    Secreted
  • Database links

  • Alternative names

    • Hepatocellular carcinoma-associated protein TB6 antibody
    • Ig alpha 1 chain C region antibody
    • Ig alpha 2 chain C region antibody
    • IGHA antibody
    • IGHA1 antibody
    • IGHA2 antibody
    • Immunoglobulin heavy constant alpha 1 antibody
    • Immunoglobulin heavy constant alpha 2 A2m marker antibody
    • Immunoglobulin heavy constant alpha 2 antibody
    • PIgR antibody
    • Poly-Ig receptor antibody
    • polymeric immunoglobulin receptor antibody
    • polymeric immunoglobulin receptor Secretory component antibody
    see all

Images

  • Paraffin-embedded human lymphoid tissue stained for IgA using ab224184 at 1 μg/mL in immunohistochemical analysis.

  • Sandwich ELISA using ab224184 as the capture antibody (100 ng/well), and Biotinylated anti-human light chains (κ+ λ) antibody ab193185 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.

  • Sandwich ELISA using ab224184 as the capture antibody (100 ng/well), and Biotinylated anti-human light chains (κ+ λ) antibody ab193185 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.

  • ELISA of human immunoglobulins shows ab224184 reacts to both Human IgA1 & IgA2. No cross reactivity with Human IgG, IgM, IgD, or IgE. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of ab224184 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

  • A titer ELISA using ab224184. The plate was coated with different amounts of human IgA. A serial dilution of ab224184 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

References

ab224184 has not yet been referenced specifically in any publications.

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