Anti-IgA Secretory Component antibody [SPM217] - BSA and Azide free (ab212330)

Overview

  • Product name

    Anti-IgA Secretory Component antibody [SPM217] - BSA and Azide free
    See all IgA Secretory Component primary antibodies
  • Description

    Mouse monoclonal [SPM217] to IgA Secretory Component - BSA and Azide free
  • Host species

    Mouse
  • Specificity

    ab212330 reacts with a reduction-resistant epitope present in both free and SIgA bound Secretory Component. It does not react with the cell lines lacking secretory component. The antibody is useful for studying the distribution and level of both free and bound secretory component.
  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Full length protein corresponding to Human IgA Secretory Component. Immunogen isolated from Human colostrum.
    Database link: Q16610

  • Positive control

    • Stomach, lung, or breast tumor; Human colon carcinoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab212330 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 2 µg/ml.
Flow Cyt Use 0.5-1µg for 106 cells.
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance

    IgA has two subclasses (IgA1 and IgA2) and can be produced as a monomeric as well as a dimeric form. The IgA dimeric form is the most prevalent and is also called secretory IgA (sIgA). The secretory component of sIgA protects the immunoglobulin from being degraded by proteolytic enzymes, thus sIgA can survive in the harsh gastrointestinal tract environment and provide protection against microbes that multiply in body secretions. sIgA can also inhibit inflammatory effects of other immunoglobulins. Secretory component is a proteolytic cleavage product of the polymeric immunoglobulin receptor which remains associated with dimeric IgA in sero-mucus secretions. Polymeric IgA binds to the polymeric immunoglobulin receptor on the basolateral surface of epithelial cells and is taken up into the cell via transcytosis. The receptor-IgA complex passes through the cellular compartments before being secreted on the luminal surface of the epithelial cells, still attached to the receptor. Proteolysis of the receptor occurs and the dimeric IgA molecule, along with the secretory component, are free to diffuse throughout the lumen. Secretory components wrap around two IgA units joined by a J chain protein fragment, resulting in a >--< configuration, with each of the two antigen binding regions of the two constituent y-shaped antibodies exposed. One identified function of secretory components is to protect IgA antibodies from degradation by the gastric acids and enzymes of the digestive system. This property is especially important in the transfer of immune system components during breastfeeding.

Images

  • Immunohistochemical analysis of formalin fixed, paraffin embedded Human colon carcinoma tissue labeling IgA Secretory Component with ab212330 at 1 ug/ml.

References

ab212330 has not yet been referenced specifically in any publications.

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