Recombinant Anti-IgA2 antibody [RM125] (ab193169)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [RM125] to IgA2
- Suitable for: ELISA, IHC-P, Sandwich ELISA
- Reacts with: Human
Overview
-
Product name
Anti-IgA2 antibody [RM125]
See all IgA2 primary antibodies -
Description
Rabbit monoclonal [RM125] to IgA2 -
Host species
Rabbit -
Tested applications
Suitable for: ELISA, IHC-P, Sandwich ELISAmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length protein corresponding to Human IgA2. Purified Native Human IgA2 from Human Myeloma Plasma
Database link: P01877
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 48% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Protein A purified -
Purification notes
Protein A affinity purified from an animal origin–free culture supernatant. -
Clonality
Monoclonal -
Clone number
RM125 -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab193169 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ELISA |
Use at an assay dependent concentration.
Use: 50ng/well – 200ng/well (for Capture); |
|
IHC-P |
Use a concentration of 0.5 - 2 µg/ml.
|
|
Sandwich ELISA |
Use at an assay dependent concentration.
|
Notes |
---|
ELISA
Use at an assay dependent concentration. Use: 50ng/well – 200ng/well (for Capture); |
IHC-P
Use a concentration of 0.5 - 2 µg/ml. |
Sandwich ELISA
Use at an assay dependent concentration. |
Target
-
Relevance
Ig alpha is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system. -
Cellular localization
Secreted -
Database links
- Entrez Gene: 3494 Human
- Omim: 147000 Human
- SwissProt: P01877 Human
-
Alternative names
- A2m marker antibody
- Ig alpha 2 chain C region antibody
- IgA constant heavy chain 2 antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgA2 antibody [RM125] (ab193169)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling IgA2 with ab193169.
-
A titer ELISA using ab193169. The plate was coated with different amounts of human IgA2. A serial dilution of ab193169 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
-
ELISA of human immunoglobulins shows ab193169 only reacted to Human IgA2. No cross reactivity with Human IgA1, IgG, IgM, IgD, or IgE. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of ab193169 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
-
Sandwich ELISA using ab193169 as the capture antibody (100ng/well), and a biotinylated anti-human light chains ( κ+ λ) antibody as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
-
Sandwich ELISA using ab193169 as the capture antibody (100ng/well), and a biotinylated anti-human light chains ( κ+ λ) antibody as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
Datasheets and documents
-
Datasheet download
References (1)
ab193169 has been referenced in 1 publication.
- Aazami H et al. Local eosinophils are associated with increased IgA subclass levels in the sinonasal mucosa of chronic rhinosinusitis with polyp patients. Allergy Asthma Clin Immunol 16:30 (2020). PubMed: 32351585