Recombinant

Recombinant Anti-IgA2 antibody [RM125] (ab193169)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [RM125] to IgA2
  • Suitable for: ELISA, IHC-P, ICC/IF, Flow Cyt, IHC-P, Sandwich ELISA
  • Reacts with: Human

Overview

  • Product name

    Anti-IgA2 antibody [RM125]
    See all IgA2 primary antibodies

  • Description

    Rabbit monoclonal [RM125] to IgA2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ELISA, IHC-P, ICC/IF, Flow Cyt, IHC-P, Sandwich ELISAmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Full length protein corresponding to Human IgA2. Purified Native Human IgA2 from Human Myeloma Plasma
    Database link: P01877

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 1% BSA, 48% PBS, 50% Glycerol
  • Concentration information loading...

  • Purity

    Protein A purified

  • Purification notes

    Protein A affinity purified from an animal origin–free culture supernatant.

  • Clonality

    Monoclonal

  • Clone number

    RM125

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab193169 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Use: 50ng/well – 200ng/well (for Capture);
0.05μg/mL – 0.2μg/mL (for Detection);
IHC-P Use a concentration of 0.5 - 2 µg/ml.
ICC/IF Use a concentration of 0.5 - 2 µg/ml.
Flow Cyt Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P Use a concentration of 0.5 - 2 µg/ml.
Sandwich ELISA Use at an assay dependent concentration.

Target

  • Relevance

    Ig alpha is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system.

  • Cellular localization

    Secreted

  • Database links

    • Alternative names

      • A2m marker antibody
      • Ig alpha 2 chain C region antibody
      • IgA constant heavy chain 2 antibody
      • IGHA 2 antibody
      • IGHA2 antibody
      • IGHA2 protein antibody
      • Immunoglobulin A2 antibody
      • Immunoglobulin Am2 antibody
      • immunoglobulin heavy constant alpha 2 (A2m marker) antibody
      • Immunoglobulin heavy constant alpha 2 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgA2 antibody [RM125] (ab193169)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgA2 antibody [RM125] (ab193169)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling IgA2 with ab193169.

    • ELISA - Anti-IgA2 antibody [RM125] (ab193169)
      ELISA - Anti-IgA2 antibody [RM125] (ab193169)

      A titer ELISA using ab193169. The plate was coated with different amounts of human IgA2. A serial dilution of ab193169 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

    • ELISA - Anti-IgA2 antibody [RM125] (ab193169)
      ELISA - Anti-IgA2 antibody [RM125] (ab193169)

      ELISA of human immunoglobulins shows ab193169 only reacted to Human IgA2. No cross reactivity with Human IgA1, IgG, IgM, IgD, or IgE. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of ab193169 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

    • Sandwich ELISA - Anti-IgA2 antibody [RM125] (ab193169)
      Sandwich ELISA - Anti-IgA2 antibody [RM125] (ab193169)

      Sandwich ELISA using ab193169 as the capture antibody (100ng/well), and a biotinylated anti-human light chains ( κ+ λ) antibody as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.

    • Sandwich ELISA - Anti-IgA2 antibody [RM125] (ab193169)
      Sandwich ELISA - Anti-IgA2 antibody [RM125] (ab193169)

      Sandwich ELISA using ab193169 as the capture antibody (100ng/well), and a biotinylated anti-human light chains ( κ+ λ) antibody as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.

    References (1)

    Publishing research using ab193169? Please let us know so that we can cite the reference in this datasheet.

    ab193169 has been referenced in 1 publication.

    • Aazami H  et al. Local eosinophils are associated with increased IgA subclass levels in the sinonasal mucosa of chronic rhinosinusitis with polyp patients. Allergy Asthma Clin Immunol 16:30 (2020). PubMed: 32351585

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