Recombinant Anti-IGBP1 antibody [EPR12315] (ab170911)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12315] to IGBP1
- Suitable for: Flow Cyt (Intra), WB, ICC/IF, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-IGBP1 antibody [EPR12315]
See all IGBP1 primary antibodies -
Description
Rabbit monoclonal [EPR12315] to IGBP1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, ICC/IF, IHC-Pmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- K562, Jurkat, HeLa and Raji cell lysates; Human pancreas tissue; Human tonsil tissue; K562 cells; HeLa cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12315 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab170911 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/1000 - 1/5000. Predicted molecular weight: 39 kDa.
|
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ICC/IF |
1/100 - 1/250.
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IHC-P |
1/100 - 1/250.
Antigen retrieval is recommended. |
Notes |
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Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/5000. Predicted molecular weight: 39 kDa. |
ICC/IF
1/100 - 1/250. |
IHC-P
1/100 - 1/250. Antigen retrieval is recommended. |
Target
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Function
Associated to surface IgM-receptor; may be involved in signal transduction. May be involved in regulation of the catalytic activity of type 2A-related serine/threonine phosphatases. -
Tissue specificity
Ubiquitously expressed with highest levels in heart, skeletal muscle and pancreas. -
Involvement in disease
Defects in IGBP1 are the cause of agenesis of the corpus callosum with mental retardation-ocular coloboma-micrognathia (ACCMRCM) [MIM:300472]. -
Sequence similarities
Belongs to the IGBP1/TAP42 family. -
Post-translational
modificationsPhosphorylated. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 3476 Human
- Entrez Gene: 18518 Mouse
- Entrez Gene: 58845 Rat
- Omim: 300139 Human
- SwissProt: P78318 Human
- SwissProt: Q61249 Mouse
- SwissProt: O08836 Rat
- Unigene: 496267 Human
see all -
Alternative names
- Alpha 4 antibody
- B cell signal transduction molecule alpha 4 antibody
- B-cell signal transduction molecule alpha 4 antibody
see all
Images
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All lanes : Anti-IGBP1 antibody [EPR12315] (ab170911) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Raji cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 39 kDa -
Immunofluorescent staining of HeLa cells labeling IGBP1 using ab170911 at 1/100 dilution (red). DAPI nuclear staining (blue).
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Intracellular flow cytometric analysis of permeabilized K562 cells labeling IGBP1 using ab170911 at 1/100 dilution (red)or a rabbit IgG negative (green).
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Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling IGBP1 using ab170911 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling IGBP1 using ab170911 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab170911 has been referenced in 1 publication.
- Wang Z et al. Targeting the transcription factor HES1 by L-menthol restores protein phosphatase 6 in keratinocytes in models of psoriasis. Nat Commun 13:7815 (2022). PubMed: 36535970