Key features and details
- Rat monoclonal [11-26] to IgD - BSA and Azide free
- Suitable for: IHC-Fr, Flow Cyt
- Reacts with: Mouse
- Isotype: IgG2a
Product nameAnti-IgD antibody [11-26] - BSA and Azide free
See all IgD primary antibodies
DescriptionRat monoclonal [11-26] to IgD - BSA and Azide free
Tested applicationsSuitable for: IHC-Fr, Flow Cytmore details
Species reactivityReacts with: Mouse
Full length protein corresponding to Mouse IgD.
- IHC-Fr: Mouse spleen tissue. Flow Cyt: C57/BL6 splenocytes.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact firstname.lastname@example.org.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Do Not Freeze.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein G purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab235126 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use a concentration of 1 µg/ml.
This product gave a positive signal in HeLa cells fixed with 10% formaldehyde (10 min).
|Flow Cyt||Use a concentration of 0.1 µg/ml.|
RelevanceLittle is known about the normal function of IgD, and few clinical signs or symptoms are associated with its absence. Individuals with low or absent levels of IgD do not appear unusually predisposed to infections. In mice, IgD may substitute for some functions of IgM when IgM is absent. IgD is expressed on peripheral B cells and is used as a pan B cell marker.
- Ig delta chain C region antibody
- IGHD antibody
- Immunoglobulin heavy constant delta antibody
C57BL/6 mouse splenocytes stained with ab235126 (right) or rat IgG2aκ (left). C57BL/6 Mouse splenocytes were incubated for 30 min on ice in 10% mouse serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab235126) or rat IgG2aκ Isotype (ab18450) (1x106 in 100µl at 0.1 µg/ml) for 30 min on ice.
The secondary antibody Goat anti-rat IgG H&L (Alexa Fluor ® 488, pre-adsorbed) (ab150165) was used at 1/2000 dilution for 30 min at 4°C.The cells were simultaneously stained with CD19 antibody.
Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable lymphocytes.
IHC image of IgD staining in a section of frozen normal mouse spleen*.
The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining.
Non-specific protein-protein interactions were blocked using TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 3% (w/v) BSA for 1 hour at room temperature. The section was then incubated with ab235126 (1µg/ml dilution) in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA overnight at +4°C. The section was then incubated with ab150167 (Goat polyclonal Secondary Antibody to Rat IgG - H&L (Alexa Fluor® 647) preabsorbed) (shown in red) and DAPI (staining nuclear DNA)(shown in blue) for 1 hour at room temperature . The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
The DAPI only control (no antibody) inset shows no autofluorescence, demonstrating that any Alexa Fluor® 647 signal is derived directly from bound ab235126.
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
*Tissue obtained from Charles River.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235126 has not yet been referenced specifically in any publications.