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  1. Link

    igd-antibody-epr6146-ab124795.pdf

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Immunology Immunoglobulins Heavy Chain IgD
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RecombinantRabMAb

Recombinant Anti-IgD antibody [EPR6146] (ab124795)

  • Datasheet
  • SDS
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgD antibody [EPR6146] (ab124795)
  • Western blot - Anti-IgD antibody [EPR6146] (ab124795)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgD antibody [EPR6146] (ab124795)
  • OI-RD Scanning - Anti-IgD antibody [EPR6146] (ab124795)
  • Western blot - Anti-IgD antibody [EPR6146] (ab124795)
  • Anti-IgD antibody [EPR6146] (ab124795)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6146] to IgD
  • Suitable for: WB, IHC-P
  • Reacts with: Human

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Overview

  • Product name

    Anti-IgD antibody [EPR6146]
    See all IgD primary antibodies
  • Description

    Rabbit monoclonal [EPR6146] to IgD
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human IgD aa 50-150. The exact sequence is proprietary.

  • Positive control

    • WB: Human tonsil lysate, Human plasma lysates IHC-P: Human tonsil tissue
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 4.00 x 10 -12 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR6146
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgD
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • B Lymphocytic Lineage

Associated products

  • Alternative Versions

    • Anti-IgD antibody [EPR6146] - BSA and Azide free (ab236778)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab124795 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
IHC-P
1/3500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/1000 - 1/2000.

Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
IHC-P
1/3500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/1000 - 1/2000.

Application notes
Is unsuitable for ICC/IF or IP.

Target

  • Relevance

    Little is known about the normal function of IgD, and few clinical signs or symptoms are associated with its absence. Individuals with low or absent levels of IgD do not appear unusually predisposed to infections. In mice, IgD may substitute for some functions of IgM when IgM is absent. IgD is expressed on peripheral B cells and is used as a pan B cell marker.
  • Cellular localization

    Cytoplasmic
  • Database links

    • Entrez Gene: 3495 Human
    • Omim: 147170 Human
    • SwissProt: P01880 Human
    • Alternative names

      • Ig delta chain C region antibody
      • IGHD antibody
      • Immunoglobulin heavy constant delta antibody

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgD antibody [EPR6146] (ab124795)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgD antibody [EPR6146] (ab124795)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling IgD with purified ab124795 at 1/3500 dilution (0.49 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    • Western blot - Anti-IgD antibody [EPR6146] (ab124795)
      Western blot - Anti-IgD antibody [EPR6146] (ab124795)
      All lanes : Anti-IgD antibody [EPR6146] (ab124795) at 1/1000 dilution (Purified)

      Lane 1 : Human plasma lysates at 15 µg


      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 42 kDa
      Observed band size: 75 kDa why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgD antibody [EPR6146] (ab124795)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgD antibody [EPR6146] (ab124795)

      ab124795, at a 1/1000 dilution, staining IgD in paraffin embedded Human tonsil tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • OI-RD Scanning - Anti-IgD antibody [EPR6146] (ab124795)
      OI-RD Scanning - Anti-IgD antibody [EPR6146] (ab124795)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Western blot - Anti-IgD antibody [EPR6146] (ab124795)
      Western blot - Anti-IgD antibody [EPR6146] (ab124795)
      Anti-IgD antibody [EPR6146] (ab124795) at 1/1000 dilution + Human tonsil lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 42 kDa

    • Anti-IgD antibody [EPR6146] (ab124795)
      Anti-IgD antibody [EPR6146] (ab124795)

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (2)

    Publishing research using ab124795? Please let us know so that we can cite the reference in this datasheet.

    ab124795 has been referenced in 2 publications.

    • Moysi E  et al. Altered immune cell follicular dynamics in HIV infection following influenza vaccination. J Clin Invest 128:3171-3185 (2018). PubMed: 29911996
    • Qu XL  et al. Establishment of a combination scoring method for diagnosis of ocular adnexal lymphoproliferative disease. PLoS One 12:e0160175 (2017). IHC-P ; Human . PubMed: 28510589

    Customer reviews and Q&As

    Show All Reviews Q&A
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    There are currently no Customer reviews or Questions for ab124795.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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