Recombinant Anti-IgD antibody [EPR6146] (ab124795)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgD antibody [EPR6146] (ab124795)](https://www.abcam.com/ps/products/124/ab124795/Images/ab124795-4-ab124795IHC1.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgD antibody [EPR6146] (ab124795)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6146] to IgD
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-IgD antibody [EPR6146]
See all IgD primary antibodies -
Description
Rabbit monoclonal [EPR6146] to IgD -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: ICC/IF or IP -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide within Human IgD aa 50-150. The exact sequence is proprietary.
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Positive control
- WB: Human tonsil lysate, Human plasma lysates IHC-P: Human tonsil tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 4.00 x 10 -12 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6146 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124795 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
|
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| IHC-P |
1/3500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified use at 1/1000 - 1/2000. |
| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 42 kDa. |
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IHC-P
1/3500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/1000 - 1/2000. |
Target
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Relevance
Little is known about the normal function of IgD, and few clinical signs or symptoms are associated with its absence. Individuals with low or absent levels of IgD do not appear unusually predisposed to infections. In mice, IgD may substitute for some functions of IgM when IgM is absent. IgD is expressed on peripheral B cells and is used as a pan B cell marker. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 3495 Human
- Omim: 147170 Human
- SwissProt: P01880 Human
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Alternative names
- Ig delta chain C region antibody
- IGHD antibody
- Immunoglobulin heavy constant delta antibody
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgD antibody [EPR6146] (ab124795)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling IgD with purified ab124795 at 1/3500 dilution (0.49 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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![Western blot - Anti-IgD antibody [EPR6146] (ab124795)](https://www.abcam.com/ps/products/124/ab124795/Images/ab124795-337514-ab124795WB1.jpg)
Western blot - Anti-IgD antibody [EPR6146] (ab124795)All lanes : Anti-IgD antibody [EPR6146] (ab124795) at 1/1000 dilution (Purified)
Lane 1 : Human plasma lysates at 15 µg
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 42 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgD antibody [EPR6146] (ab124795)](https://www.abcam.com/ps/products/124/ab124795/Images/IGHD-Primary-antibodies-ab124795-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgD antibody [EPR6146] (ab124795)ab124795, at a 1/1000 dilution, staining IgD in paraffin embedded Human tonsil tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-IgD antibody [EPR6146] (ab124795)](https://www.abcam.com/ps/products/124/ab124795/Images/IGHD-Primary-antibodies-ab124795-3.jpg)
OI-RD Scanning - Anti-IgD antibody [EPR6146] (ab124795) -
![Western blot - Anti-IgD antibody [EPR6146] (ab124795)](https://www.abcam.com/ps/products/124/ab124795/Images/IGHD-Primary-antibodies-ab124795-1.jpg)
Western blot - Anti-IgD antibody [EPR6146] (ab124795)Anti-IgD antibody [EPR6146] (ab124795) at 1/1000 dilution + Human tonsil lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 42 kDa -
![Anti-IgD antibody [EPR6146] (ab124795)](https://www.abcam.com/ps/products/124/ab124795/Images/ab124795-6-benefits-of-recombinant-antibodies.png)
Anti-IgD antibody [EPR6146] (ab124795)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab124795 has been referenced in 2 publications.
- Moysi E et al. Altered immune cell follicular dynamics in HIV infection following influenza vaccination. J Clin Invest 128:3171-3185 (2018). PubMed: 29911996
- Qu XL et al. Establishment of a combination scoring method for diagnosis of ocular adnexal lymphoproliferative disease. PLoS One 12:e0160175 (2017). IHC-P ; Human . PubMed: 28510589