RecombinantRabMAb

Recombinant Anti-IgD antibody [EPR6146] (ab124795)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6146] to IgD
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-IgD antibody [EPR6146]
    See all IgD primary antibodies

  • Description

    Rabbit monoclonal [EPR6146] to IgD

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC/IF or IP

  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human IgD aa 50-150. The exact sequence is proprietary.

  • Positive control

    • WB: Human tonsil lysate, Human plasma lysates IHC-P: Human tonsil tissue

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab124795 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
IHC-P 1/3500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/1000 - 1/2000.
  • Application notes
    Is unsuitable for ICC/IF or IP.

    • Target

    • Relevance

      Little is known about the normal function of IgD, and few clinical signs or symptoms are associated with its absence. Individuals with low or absent levels of IgD do not appear unusually predisposed to infections. In mice, IgD may substitute for some functions of IgM when IgM is absent. IgD is expressed on peripheral B cells and is used as a pan B cell marker.

    • Cellular localization

      Cytoplasmic

    • Database links

      • Alternative names

        • Ig delta chain C region antibody
        • IGHD antibody
        • Immunoglobulin heavy constant delta antibody

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgD antibody [EPR6146] (ab124795)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgD antibody [EPR6146] (ab124795)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling IgD with purified ab124795 at 1/3500 dilution (0.49 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

      • Western blot - Anti-IgD antibody [EPR6146] (ab124795)
        Western blot - Anti-IgD antibody [EPR6146] (ab124795)
        All lanes : Anti-IgD antibody [EPR6146] (ab124795) at 1/1000 dilution (Purified)

        Lane 1 : Human plasma lysates at 15 µg


        Secondary
        All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

        Predicted band size: 42 kDa
        Observed band size: 75 kDa
        why is the actual band size different from the predicted?

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgD antibody [EPR6146] (ab124795)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgD antibody [EPR6146] (ab124795)

        ab124795, at a 1/1000 dilution, staining IgD in paraffin embedded Human tonsil tissue by Immunohistochemistry.

        Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

      • Other - Anti-IgD antibody [EPR6146] (ab124795)
        Other - Anti-IgD antibody [EPR6146] (ab124795)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD
      • Western blot - Anti-IgD antibody [EPR6146] (ab124795)
        Western blot - Anti-IgD antibody [EPR6146] (ab124795)
        Anti-IgD antibody [EPR6146] (ab124795) at 1/1000 dilution + Human tonsil lysate at 10 µg

        Secondary
        HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 42 kDa

      References (1)

      Publishing research using ab124795? Please let us know so that we can cite the reference in this datasheet.

      ab124795 has been referenced in 1 publication.

      • Qu XL  et al. Establishment of a combination scoring method for diagnosis of ocular adnexal lymphoproliferative disease. PLoS One 12:e0160175 (2017). IHC-P ; Human . PubMed: 28510589

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