Recombinant Biotin Anti-IgD antibody [RM123] (ab224182)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Biotin Rabbit monoclonal [RM123] to IgD
- Suitable for: Sandwich ELISA, ELISA, IHC
- Reacts with: Human
- Conjugation: Biotin
Related conjugates and formulations
Overview
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Product name
Biotin Anti-IgD antibody [RM123]
See all IgD primary antibodies -
Description
Biotin Rabbit monoclonal [RM123] to IgD -
Host species
Rabbit -
Conjugation
Biotin -
Specificity
ab224182 reacts to human IgD. No cross reactivity with human IgG, IgM, IgA, or IgE. -
Tested applications
Suitable for: Sandwich ELISA, ELISA, IHCmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified) corresponding to Human IgD aa 1 to the C-terminus.
Database link: P01880
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
ab224182 was purified from an animal origin–free culture supernatant . -
Clonality
Monoclonal -
Clone number
RM123 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab224182 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Sandwich ELISA |
Use at an assay dependent concentration.
Use: 25 - 200 ng/well (for capture); 0.01 - 0.1 µg/ml (for detection). |
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ELISA |
Use at an assay dependent concentration.
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IHC |
Use a concentration of 0.5 - 2 µg/ml.
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Notes |
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Sandwich ELISA
Use at an assay dependent concentration. Use: 25 - 200 ng/well (for capture); 0.01 - 0.1 µg/ml (for detection). |
ELISA
Use at an assay dependent concentration. |
IHC
Use a concentration of 0.5 - 2 µg/ml. |
Target
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Relevance
Little is known about the normal function of IgD, and few clinical signs or symptoms are associated with its absence. Individuals with low or absent levels of IgD do not appear unusually predisposed to infections. In mice, IgD may substitute for some functions of IgM when IgM is absent. IgD is expressed on peripheral B cells and is used as a pan B cell marker. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 3495 Human
- Omim: 147170 Human
- SwissProt: P01880 Human
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Alternative names
- Ig delta chain C region antibody
- IGHD antibody
- Immunoglobulin heavy constant delta antibody
Images
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A titre ELISA using ab224182. The plate was coated with different amounts of human IgD from plasma. A serial dilution of ab224182 was used as primary antibody. An alkaline phosphatase-conjugated Anti-Rabbit IgG was used as secondary antibody.
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ELISA of human immunoglobulins shows that ab224182 reacts to IgD from human plasma and IgDκ from human melanoma. No cross reactivity with human IgG, IgM, IgA or IgE. The plate was coated with 50 ng/well of the different immunoglobulins. 200 ng/ml, 50 ng/ml or 10 ng/ml of ab224182 was used as the primary antibody. An alkaline phosphatase-conjugated Anti-Rabbit IgG was used as secondary antibody.
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Human lymphoid tissue stained for IgD with ab224182 (2 μg/ml) in immunohistochemical analysis.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab224182 has not yet been referenced specifically in any publications.