Overview

  • Product name
    Anti-IgE antibody [4C3]
    See all IgE primary antibodies
  • Description
    Mouse monoclonal [4C3] to IgE
  • Host species
    Mouse
  • Specificity
    ab106494 is specific for Human IgE. It reacts with IgE purified from blood but does not cross-react with Human anti-NP IgG1, IgG2, IgG3, IgG4, IgA and IgM
  • Tested applications
    Suitable for: WB, ELISA, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse
  • Immunogen

    Mouse-Human chimeric anti-NP IgE

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    4C3
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab106494 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 47 kDa.
ELISA Use a concentration of 1 µg/ml. Can be paired for ELISA with Mouse monoclonal [1A2] to IgE (HRP) (ab106493). as primary antibody
Sandwich ELISA Use a concentration of 5 µg/ml. for coating plates. Use ab106493 for detection.

Target

  • Relevance
    IgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion, but it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies - and the life threatening respiratory distress of asthma and anaphylactic shock.
  • Cellular localization
    Cell Membrane
  • Database links
    • Alternative names
      • Igh2 antibody
      • IGHE antibody
      • IGHEP1 antibody
      • Immunoglobulin heavy constant epsilon antibody

    Images

    • Sandwich ELISA - Anti-IgE antibody [4C3] (ab106494)
      Sandwich ELISA - Anti-IgE antibody [4C3] (ab106494)
      ab106494 used at 5 µg/ml for coating ELISA plates to capture Human IgE. Detection utilised ab106493 at 1/1000 dilution.

    References

    ab106494 has not yet been referenced specifically in any publications.

    Publishing research using ab106494? Please let us know so that we can cite the reference in this datasheet.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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