Anti-IgE antibody [4C3] (ab106494)

Submit a review Submit a question References (1)
Sandwich ELISA - Anti-IgE antibody [4C3] (ab106494)

    Key features and details

    • Mouse monoclonal [4C3] to IgE
    • Suitable for: Sandwich ELISA
    • Reacts with: Human
    • Isotype: IgG1

    Get better batch-to-batch reproducibility with a recombinant antibody

    Product image
    Anti-IgE antibody [RM122] (ab195580)
    • Research with confidence – consistent and reproducible results with every batch
    • Long-term and scalable supply – powered by recombinant technology for fast production
    • Success from the first experiment – confirmed specificity through extensive validation
    • Ethical standards compliant – production is animal-free

    Overview

    • Product name

      Anti-IgE antibody [4C3]
      See all IgE primary antibodies

    • Description

      Mouse monoclonal [4C3] to IgE

    • Host species

      Mouse

    • Specificity

      ab106494 is specific for Human IgE. It reacts with IgE purified from blood but does not cross-react with Human anti-IgG1, IgG2, IgG3, IgG4, IgA and IgM

    • Tested applications

      Suitable for: Sandwich ELISAmore details

    • Species reactivity

      Reacts with: Human
      Does not react with: Mouse

    • Immunogen

      Fusion protein corresponding to Human IgE.
      Database link: P01854

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • Storage buffer

      Preservative: 0.1% Sodium azide
      Constituent: PBS
    • Concentration information loading...

    • Purity

      Protein A purified

    • Clonality

      Monoclonal

    • Clone number

      4C3

    • Isotype

      IgG1

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab106494 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Sandwich ELISA
    Use a concentration of 5 µg/ml. for coating plates. Use ab106493 for detection.
    Notes
    Sandwich ELISA
    Use a concentration of 5 µg/ml. for coating plates. Use ab106493 for detection.

    Target

    • Relevance

      IgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion, but it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies - and the life threatening respiratory distress of asthma and anaphylactic shock.

    • Cellular localization

      Cell Membrane

    • Database links

      • Alternative names

        • Igh2 antibody
        • IGHE antibody
        • IGHEP1 antibody
        • Immunoglobulin heavy constant epsilon antibody

      Images

      • Sandwich ELISA - Anti-IgE antibody [4C3] (ab106494)
        Sandwich ELISA - Anti-IgE antibody [4C3] (ab106494)
        ab106494 used at 5 µg/ml for coating ELISA plates to capture Human IgE. Detection utilised ab106493 at 1/1000 dilution.

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (1)

      Publishing research using ab106494? Please let us know so that we can cite the reference in this datasheet.

      ab106494 has been referenced in 1 publication.

      • Leung S  et al. Multiplexed immunoassay approach to characterize antidrug antibody like specific reactivity. Bioanalysis 11:703-712 (2019). PubMed: 30994012

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      There are currently no Customer reviews or Questions for ab106494.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com