Anti-IgE antibody [4C3] (ab106494)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-IgE antibody [4C3]
See all IgE primary antibodies -
Description
Mouse monoclonal [4C3] to IgE -
Host species
Mouse -
Specificity
ab106494 is specific for Human IgE. It reacts with IgE purified from blood but does not cross-react with Human anti-IgG1, IgG2, IgG3, IgG4, IgA and IgM
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Tested applications
Suitable for: WB, ELISA, Sandwich ELISAmore details -
Species reactivity
Reacts with: Human
Does not react with: Mouse -
Immunogen
Fusion protein corresponding to Human IgE.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
4C3 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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ELISA pair antibody
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab106494 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 47 kDa. | |
| ELISA | Use a concentration of 1 µg/ml. Can be paired for ELISA with Mouse monoclonal [1A2] to IgE (HRP) (ab106493). as primary antibody | |
| Sandwich ELISA | Use a concentration of 5 µg/ml. for coating plates. Use ab106493 for detection. |
Target
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Relevance
IgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion, but it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies - and the life threatening respiratory distress of asthma and anaphylactic shock. -
Cellular localization
Cell Membrane -
Database links
- Entrez Gene: 3497 Human
- Omim: 147180 Human
- SwissProt: P01854 Human
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Alternative names
- Igh2 antibody
- IGHE antibody
- IGHEP1 antibody
- Immunoglobulin heavy constant epsilon antibody
Images
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![Sandwich ELISA - Anti-IgE antibody [4C3] (ab106494)](https://www.abcam.com/ps/products/106/ab106494/Images/ab106494-118874-anti-ige-antibody-4c3-sandwich-elisa.jpg)
Sandwich ELISA - Anti-IgE antibody [4C3] (ab106494)ab106494 used at 5 µg/ml for coating ELISA plates to capture Human IgE. Detection utilised ab106493 at 1/1000 dilution.
Protocols
References
This product has been referenced in:
- Leung S et al. Multiplexed immunoassay approach to characterize antidrug antibody like specific reactivity. Bioanalysis 11:703-712 (2019). Read more (PubMed: 30994012) »
