Overview

  • Product name

    Anti-IgE antibody [4H10] (FITC)
    See all IgE primary antibodies
  • Description

    Mouse monoclonal [4H10] to IgE (FITC)
  • Host species

    Mouse
  • Conjugation

    FITC. Ex: 493nm, Em: 528nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Purified human IgE.

  • General notes

    The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.097% Sodium azide
    Constituent: 0.2% BSA

    0.2% (w/v) high-grade protease free BSA
  • Concentration information loading...
  • Purity

    Size exclusion
  • Clonality

    Monoclonal
  • Clone number

    4H10
  • Isotype

    IgG1
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab77239 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use a concentration of 2 - 5 µg/ml.

ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Relevance

    IgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion, but it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies - and the life threatening respiratory distress of asthma and anaphylactic shock.
  • Cellular localization

    Cell Membrane
  • Database links

  • Alternative names

    • Igh2 antibody
    • IGHE antibody
    • IGHEP1 antibody
    • Immunoglobulin heavy constant epsilon antibody

Images

  • ab77239 staining IgE in Human basophil granulocytes by Flow Cytometry. Cells were peripheral blood cells, separated by Ficoll density separation, followed by magnetic isolation. The sample was incubated with the primary antibody (3μg/ml in PBS with Human IgG (Fc-block)) for 45 minutes at 4°C.

    See Abreview

References

This product has been referenced in:

  • Gervais FG  et al. Pharmacological characterization of MK-7246, a potent and selective CRTH2 (chemoattractant receptor-homologous molecule expressed on T-helper type 2 cells) antagonist. Mol Pharmacol 79:69-76 (2011). Flow Cyt ; Human . Read more (PubMed: 20943773) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Flow Cytometry
Fixation
no fixation
Permeabilization
No
Sample
Human Cell (human basophil granulocytes)
Specification
human basophil granulocytes
Gating Strategy
no gate
Preparation
Cell harvesting/tissue preparation method: peripheral blood cells, separated by Ficoll density separation, followed by magnetic isolation
Sample buffer: PBS with human IgG (Fc-block)

Abcam user community

Verified customer

Submitted Aug 15 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up