Overview

  • Product name
    Anti-IgE antibody [5D4]
    See all IgE primary antibodies
  • Description
    Mouse monoclonal [5D4] to IgE
  • Host species
    Mouse
  • Specificity
    Specific for IgE Ce2-domain specific
  • Tested applications
    Suitable for: ELISA, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein (purified) corresponding to Human IgE. IgE from myelomatous human serum.

  • General notes
    Concentration varies from lot to lot and can be provided on request.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    Purified by chromatography on protein A Sepharose. Purity is tested by electrophoresis.
  • Clonality
    Monoclonal
  • Clone number
    5D4
  • Myeloma
    Sp2/0
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7295 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution. Ab7295 can be used for the detection of total IgE levels and IgE antibodies by ELISA
Sandwich ELISA Use at an assay dependent dilution. Can be paired for Sandwich ELISA with Mouse monoclonal [4F4] to IgE (HRP) (ab7294). Can be used as Capture antibody with recommended pair.

Target

  • Relevance
    IgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion, but it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies - and the life threatening respiratory distress of asthma and anaphylactic shock.
  • Cellular localization
    Cell Membrane
  • Database links
  • Alternative names
    • Igh2 antibody
    • IGHE antibody
    • IGHEP1 antibody
    • Immunoglobulin heavy constant epsilon antibody

Images

  • Standard curve: Capture anitbody (ab7295) at 5 ug/ml in PBS, human IgE from 100 ng/ml to 1.56 ng/ml, detection antibody (ab7294, HRP conjugated) at 0.7 ug/ml concentration.

References

This product has been referenced in:
See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for contacting us.   I have received the following information from the lab: We are using ab7295 according to the datasheet recommendation as the capture antibody, not as detection antibody. The detection antibody ab7295 was used with the HRP-conjugated antibody ab7294. With this antibody pair we get the standard curve that is attached to this email.    We have used very standard internal protocol without any optimization: capture antibody ab7295 at 5 ug/ml in PBS, human IgE from 100 ng/ml to 1.56 ng/ml, detection antibody ab7294 (HRP) at 0.7 ug/ml concentration.    Unfortunately we don’t have any results using ab7295 as detection antibody. I am sorry for this inconvenience. However, it seems that levels of IgE much lower than 5 ug/mL or even 0.37 ug/mL are detectable with ab7295 used as capture antibody. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. 

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Answer

Thank you for your enquiry. In our internal assay for anti-IgE MAb testing, highly purified myelomatous IgE(VL) was adsorbed on solid phase from solution (5 microgram/ml) in bicarbonate buffer pH 9.5. Ab7295 was applied for 60-120 min onto antigen and after washing HRP labeled affinity purified rabbit anti-mouse Ig antibody in TRIS-Tween buffer was used as developing reagent. The value 1:1 000 000 means the end-point of titration in conditions mentioned above. Generally ab7295 is recommended as capture antibody. Its concentration for immobilization onto solid phase may be 5-10 microgram/ml depending on kind of plastic plates used by customer. I hope this information helps. Please do not hesitate to contact us again if you need anything further.

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Answer

Thank you for your enquiry and for completing our technical questionnaire. I am sorry to hear that you have been having difficulties with this antibody. I would greatly appreciate it if you could tell me of the concentration that you have been using this antibody at. Our datasheet states a 1/ 1 x 10^6 dilution. However, I consider this exceptionally dilute even by its application using ELISA. Your details of the concentration that you have been using was unfortunately lost in the formating of the e-mail. Please can you therefore tell me whether you have been using this antibody to coat the wells at 1ng/ml or 1ug/ml. I look forward to hearing from you.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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