Overview

  • Product name
    Anti-IgE antibody [BE5] (FITC)
    See all IgE primary antibodies
  • Description
    Mouse monoclonal [BE5] to IgE (FITC)
  • Host species
    Mouse
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Specificity
    This antibody reacts with IgE
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein (purified) corresponding to Human IgE.

  • General notes

    The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.097% Sodium azide
    Constituents: PBS, 0.2% BSA
  • Concentration information loading...
  • Purity
    Size exclusion
  • Clonality
    Monoclonal
  • Clone number
    BE5
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab49434 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use a concentration of 5 µg/ml.

ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Relevance
    IgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion, but it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies - and the life threatening respiratory distress of asthma and anaphylactic shock.
  • Cellular localization
    Cell Membrane
  • Database links
  • Alternative names
    • Igh2 antibody
    • IGHE antibody
    • IGHEP1 antibody
    • Immunoglobulin heavy constant epsilon antibody

References

This product has been referenced in:
  • Gervais FG  et al. Pharmacological characterization of MK-7246, a potent and selective CRTH2 (chemoattractant receptor-homologous molecule expressed on T-helper type 2 cells) antagonist. Mol Pharmacol 79:69-76 (2011). Flow Cyt ; Human . Read more (PubMed: 20943773) »
See 1 Publication for this product

Customer reviews and Q&As

Answer

Unfortunately, having consulted with the source of this antibody, the epitope of the antibody is not known. However, it has been established that the antibody detects a different epitope to that of clones 4G7 and 4H10 (4G7: ab774 and 4H10: ab774 and ab77121 PE-dynamics conjugated). If you find there is an overlap with ab49434 and your other antibody you could change to using one of these alternatives. Otherwise we have a few anti-human IgE of which the epitope is know. ab7294 and ab7295 are specific for the Ce3 and Ce2 region respectively. ab7378 is specific for the Fc region and ab7382 is specific for the epsilon chain. I hope this information has been of help to you. If you have any further questions please do not hesitate to ask.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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