Anti-IgE antibody [BE5] (Phycoerythrin) (ab77137)

Overview

  • Product name
    Anti-IgE antibody [BE5] (Phycoerythrin)
    See all IgE primary antibodies
  • Description
    Mouse monoclonal [BE5] to IgE (Phycoerythrin)
  • Host species
    Mouse
  • Conjugation
    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applications
    Suitable for: Flow Cyt, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein (purified) corresponding to Human IgE.

  • Positive control
    • Basophil from normal (heparin treated) whole blood stimulated with a combination of IL-3 and Goat anti-IgE polyclonal antibody.
  • General notes

    The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 15mM Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity
    >95% by SDS-PAGE
  • Purification notes
    The conjugate is purified by size exclusion chromatography.
  • Clonality
    Monoclonal
  • Clone number
    BE5
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77137 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use a concentration of 5 - 10 µg/ml.

ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

ELISA Use at an assay dependent concentration.

Target

  • Relevance
    IgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion, but it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies - and the life threatening respiratory distress of asthma and anaphylactic shock.
  • Cellular localization
    Cell Membrane
  • Database links
  • Alternative names
    • Igh2 antibody
    • IGHE antibody
    • IGHEP1 antibody
    • Immunoglobulin heavy constant epsilon antibody

Images

  • Analysis by flow cytometry of ab77137 staining IgE in human peripheral blood cells.

References

ab77137 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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