Overview

  • Product name

    Anti-IgE antibody, prediluted
    See all IgE primary antibodies
  • Description

    Rabbit polyclonal to IgE, prediluted
  • Host species

    Rabbit
  • Specificity

    This antibody recognizes IgE, epsilon heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), mu (IgM), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes.
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length IgE isolated from a pool of normal human sera.

  • Positive control

    • Human Tonsil tissue
  • General notes


    This antibody is useful in the identification of leukemias, plasmacytomas and certain non-Hodgkin’s lymphomas.

Properties

  • Form

    Prediluted
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.60
    Preservative: 0.0975% Sodium azide
    Constituent: 0.79% Tris HCl

    Containing antibody stabilizer
  • Concentration information loading...
  • Purity

    Protein A purified
  • Primary antibody notes

    This antibody is useful in the identification of leukemias, plasmacytomas and certain non-Hodgkin’s lymphomas.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab75673 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Ready to use.

Target

  • Relevance

    IgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion, but it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies - and the life threatening respiratory distress of asthma and anaphylactic shock.
  • Cellular localization

    Cell Membrane
  • Database links

  • Alternative names

    • Igh2 antibody
    • IGHE antibody
    • IGHEP1 antibody
    • Immunoglobulin heavy constant epsilon antibody

Images

  • Formalin-fixed, paraffin-embedded human tonsil stained with ab75673, using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of IgE-secreting cells.

References

This product has been referenced in:

  • Ningyan G  et al. The role of macrophage migration inhibitory factor in mast cell-stimulated fibroblast proliferation and collagen production. PLoS One 10:e0122482 (2015). Read more (PubMed: 25826375) »
  • Motegi S  et al. Elevated serum levels of TARC/CCL17, Eotaxin-3/CCL26 and VEGF in a patient with Kimura's disease and prurigo-like eruption. Acta Derm Venereol 94:112-3 (2014). Read more (PubMed: 23756566) »
See all 2 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (duodenum - normal and celiac dz)
Specification
duodenum - normal and celiac dz
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA pH9.0, 100C 20 min
Permeabilization
No

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Verified customer

Submitted Dec 09 2010

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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